FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003685698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003685698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.25957170668397145No Hit
GGTAGGGGGGGGAGTTTAAACTTGTTCCCTTTACTTGCCCAGGAACCCAT20.12978585334198572No Hit
GTGGTTAGGTTCTGAAGGAGTTCAGAAAATGATGCTTTTTCTGAAAGCAAACATGATGCTTTGGTGTGCTGATTA20.12978585334198572No Hit
GGGATGGGGGGCAATGCTAGTAAAGAACAATGCTAGTTTCACAATGGTAG20.12978585334198572No Hit
TGGTGGGTGGGGTCATAAAGGTTGAACACAAAGGCATGCTTTTCAAGGCC20.12978585334198572No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG20.12978585334198572Illumina Single End PCR Primer 1 (100% over 32bp)
AGTAGTGTTAGCTGCATTATTTTTCAATTTAAATATGGTGTTGTCATAGA20.12978585334198572No Hit
CGGGGGCGGGTGTGGACACAGTATTAACCCATTAGCTCATGGACCTTGGG20.12978585334198572No Hit
GTGGGGGGGGTGTCCTGTGCATTGTAGGATGTTTAGCACCATCTCGGGGC20.12978585334198572No Hit
TGGTGGGTTGTAACAACCATGGAATGACTTAACCACTGAGAACACAATCCATAGAATAACAACAACAAAAGTCAC20.12978585334198572No Hit
TGTAGGGGGGTTGCAGAATTTAGCAGGTCATTATAGACATTTGAGAGTAC20.12978585334198572No Hit
CTGAGGTGTGGTAGGATAAGAGGAGGAACTTCCTAAGGCTGGAGAAGGCG20.12978585334198572No Hit
GTGAGGGGGGTAAGAAAAAAATTTGAAGGGATAATGGCTGACATTTCTGC20.12978585334198572No Hit
GGGAGGTGGGTCTGTCGTTCTAGATCTTTCCAGCTGACTCTGGGGAAAGGATGCAGGGAGAAGAAAAGCTCCTTC20.12978585334198572No Hit
CGGCTGATGGTGGTTCCCCAGGAACTTAGGGACACCTGTGTGCAGGGCTG20.12978585334198572No Hit
AGGGGGTGTTGGCTAGTTACCTCTCTCTTGCCTTGTCTGTTTCCAGATAT20.12978585334198572No Hit
CTGGTGCTGGTGATGTACAGATGGGTTTTTGGTGTGGATGTCCTTTCTGT20.12978585334198572No Hit
CGGGGTTTGGGGTACAGTTGATCCCATCACCCATGTAGTGAGCATAGCACCCAATAGTTTTTCAACCCTTGCCCT20.12978585334198572No Hit
TGTGGGCTGCTATTTCATCTTCAATACAGTAAAGACATACTTGAGCAATC20.12978585334198572No Hit
ATTCGTGGGAGTTGAGCAAGAACAAGGGGTACAGAACTTACTTAAATAAA20.12978585334198572No Hit
TTTGGTCTGTGTTATGGAATACTATTTTACAAACTTTTTTTGACTGTAAT20.12978585334198572No Hit
GGGTGGCTGATGGATGAAGAAACAAGACCCAATGATCTATTGCTTGCAAG20.12978585334198572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)