FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003685745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003685745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21531
Sequences flagged as poor quality0
Sequence length68-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10775.0020900097533785No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATCTCAGGATCTCGTATGCCGT1980.9196042914866935TruSeq Adapter, Index 15 (97% over 35bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.8360039013515397No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.7384701128605267No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.7013143839115694No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.48302447633644513No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.3947796200826715No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.35297942501509455No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.3390460266592355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)