FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003686161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5286797
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG169250.32013712650589765No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC142980.2704473048615258No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC135670.25662040740357533No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC134290.2540101312760827No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC115320.21812829204525913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111330.21058118932881287No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA103640.19603552018358184No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT90530.17123789697240124No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC88130.16669828631589223No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC83750.15841349686776324No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA69540.1315352187723493No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT68500.12956805415452874No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC64270.12156699037243156No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT61210.11577898678538252No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG58400.11046385930838652No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG54680.10342746279079754No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA53610.10140355303977057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.10009841497602423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)