FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003686336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7657945
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136140.17777615274071568No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC110370.1441248272219244Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC108820.14210078552405378Clontech Universal Primer Mix Long (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN105980.13839221880021338No Hit
GTACGGGAGAATAAAAACAGAAAAATGGCGACGGAAAAAAAAAAAAAAAA82930.10829276000284672No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA82790.10810994333336163Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT81440.10634706830618397Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)