FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003686517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length75-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGT13.4482758620689653No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTAT13.4482758620689653No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT13.4482758620689653No Hit
AGTAGTTATAGGCTGACAAGTCCACTTTTGGCTTACCATGGAGATCTGCTCCAACACATGCTAGTCGTTAGATCA13.4482758620689653No Hit
GTTAGTGAATTAGAAAGAGTTAAATAAATTTAAAAATCCCCACTATTTCT13.4482758620689653No Hit
CTTGAAAGCATCCATGTGTGGACTCAAGTCCTTACCTCTTCCGGAGATGT13.4482758620689653No Hit
CTTTCCTGATGATCCACCCACATTTATCTTCACCAACATGCAAGTTCTTT13.4482758620689653No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA13.4482758620689653No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA13.4482758620689653No Hit
AAGTTTGACCGAGTGTCTCGACTGCATTGTGAGAGTGAATCTTTCAAGATGGATCTAATCTTAGATGTAAACATT13.4482758620689653No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG13.4482758620689653No Hit
GTACTGCATTGATATGCTGAGACTAATTGCCAAATTTTAAAATTATCTAC13.4482758620689653No Hit
GAATATCTGTTCACTTTTCAATCCCAATTCTGTCCCAATCTTACCAGATG13.4482758620689653No Hit
TTTACATACTCTGCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGAC13.4482758620689653No Hit
GTGAAGGGAGCCCGGGCAGCCACCGTCTCCAGAGCCCTGGGCAGCATTTTCCAGCAAGATGTACACAATCTTTTG13.4482758620689653No Hit
GACCTTCCCAGTGCCAACGTCCACATAGGACAGGGTGTGCTTCCTCCAGTGCTCCTCAAAGGGCTTCTTCTGTTG13.4482758620689653No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT13.4482758620689653No Hit
TCTCTATTATGTGGTGTATTTACCGTTGAACTTGGCTCTGCACACCACCT13.4482758620689653No Hit
CCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAA13.4482758620689653No Hit
ACTTTGGCAGGATGTCTGGGGCCTCACCAGCAGGAGCGCGTGCAAGCCGGGCAGGCGGTCCACCTAGACCCACAG13.4482758620689653No Hit
GCTACAAGGGCTAAGAACTTGATGCTTTTTCTCCAGTACTTGGTACAGAA13.4482758620689653No Hit
AAAAAATAGTTACAAACAAAAATAGGAACAACAAAATGATAATTCAACAC13.4482758620689653No Hit
ATGCCAGTTAGGCACATTCTTGTAAGTAACTCTCGATGTTACAACAAACA13.4482758620689653No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA13.4482758620689653No Hit
CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG13.4482758620689653No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAACGCCCTAACTTAATGGACGGGAGGTAT13.4482758620689653No Hit
AGCATGTACATCCCTAATTAATTTAAAGCCCTCTGTAGAGAAATTAAACT13.4482758620689653No Hit
AATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATAT13.4482758620689653No Hit
GTTAAAGGTTGGATTTAAGTTGAAGTTTTTCGGAGACGGTTATGAGAAGA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)