FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003686520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4891618
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA955671.9536889430041349Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA507981.0384702975579858Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT286670.5860433091872669Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATAAACGCAGA135430.27686135753037133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATAAACGCA132520.27091240567027103Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG131220.2682547983100888Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA121240.24785255103730502Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACGGGACATGAACTTAAAATATTTTTATGTCTTCTTTATGAATAACTAAGAATTGAACCTATATTGTTGATT117310.23981839955613868No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACGGGACATGAACTTAAAATATT113390.23180469120851221Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACTCAGA106650.2180260192026442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA106100.21690164685795169Clontech SMART CDS Primer II A (96% over 26bp)
CAACAATATAGGTTCAATTCTTAGTTATTCATAAAGAAGACATAAAAATATTTTAAGTTCATGTCCCGTACTCTG104910.21446891396670795No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG89160.18227097864142294Clontech Universal Primer Mix Long (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86770.17738506972539558No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG85480.1747479054987532Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGGACATGAACTTAAAATATTTTTATGTCTTCTTTATGAATAAC83770.1712521296634365No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT78120.15970175921341365Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACTCA78050.15955865727863458Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA77280.1579845359960651Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAAGCAGA75150.15363014855207419Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAATCA64090.13102004285698515Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT64030.13089738405574597Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA62710.12819889042848398Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA60430.12353785598139512Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAAGCA59230.1210846799566115Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGGG58690.11998075074545886Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTGTTGATTCTAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55830.11413401455305791No Hit
GTACGGGCAGTGGTATCAACGCAGAGTACGGGACATGAACTTAAAATATT54780.11198748553137222Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC53690.10975918397552711Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51690.10567055726755442Clontech SMART CDS Primer II A (100% over 26bp)
ATATAGGTTCAATTCTTAGTTATTCATAAAGAAGACATAAAAATATTTTA50510.10325826750985052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)