FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003686679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7294499
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC560450.7683187015311127Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC390660.5355542580785877Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA263650.3614367484319348Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA212950.29193231776438655Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG174030.23857704278251324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA148730.20389337225215878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134230.18401537926045367No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC118430.16235522137983707Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG105560.1447117889796133Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGGTGATAC85360.11701968839806544Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA85030.11656729269549561Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA80580.11046680519114473Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG79940.10958943170737291Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)