FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003686680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003686680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7294499
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC328830.4507917541698203Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC245050.3359380815598165Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA207530.284502061073694Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG161250.22105699102844484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138350.18966347106223472No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA136860.18762083592032847Clontech Universal Primer Mix Long (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121580.16667354399527645No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA107830.14782372305486643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACAATTCTATTATAC105730.14494484131124016Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)