FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003935788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003935788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61965
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC41766.739288307915759No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC33285.370773823932865No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC29874.820463164689745No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC28934.66876462519164No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC24563.963527797950456No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC23693.8231259582022106No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC20703.340595497458242No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC17702.8564512224642944No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT16572.674090212216574No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC13632.199628822722505No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT12492.0156539982248045No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT12031.9414185427257324No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC9531.5379649802307753No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC9421.5202130234809974No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8851.4282256112321472No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT8811.421770354232228No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC7191.1603324457354958No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC6971.1248285322359397No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC6801.0973936899862824No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC6641.0715726619866053No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT6611.0667312192366658No Hit
GTGCCAGCCGCCGCGGTAAGACGTAGGTCACAAGCGTTGTCCGGAATTAC6140.990881949487614No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC5820.9392398934882594No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC5600.9037359799887034No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT5100.823045267489712No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC5100.823045267489712No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT5020.8101347534898733No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC4920.793996610990075No Hit
GTGCCAGCAGCCGCGGTAAGACGTAGGTCACAAGCGTTGTCCGGAATTAC4880.7875413539901557No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC4850.7826999112402162No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT4770.7697893972403776No Hit
GTGTCAGCCGCCGCGGTAAGACGTAGGTCACAAGCGTTGTCCGGAATTAC4540.7326716694908416No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4320.6971677559912853No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4250.6858710562414266No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC4190.6761881707415477No Hit
GTGTCAGCAGCCGCGGTAAGACGTAGGTCACAAGCGTTGTCCGGAATTAC3950.6374566287420318No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3900.6293875574921327No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3890.6277737432421528No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC3840.6197046719922537No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3830.6180908577422739No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3510.5664488017429194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT3490.5632211732429597No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3490.5632211732429597No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3150.5083514887436456No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC3040.4905995319938675No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC3010.485758089243928No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2950.4760752037440491No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2830.4567094327442911No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2700.4357298474945534No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2550.411522633744856No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT2520.40668119099491645No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2420.39054304849511823No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT2180.3518115064956024No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2060.33244573549584444No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2050.3308319212458646No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1920.3098523359961268No Hit
GTGCCAGCCGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC1880.30339707899620755No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1840.2969418219962882No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT1830.2953280077463084No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1830.2953280077463084No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1710.27596223674655046No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1690.2727346082465908No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1640.2646655369966917No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT1590.25659646574679257No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1550.25014120874687323No Hit
GTGTCAGCCGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC1430.23077543774711531No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1420.22916162349713548No Hit
GTGCCAGCAGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC1420.22916162349713548No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1330.21463729524731703No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1300.20979585249737753No Hit
GTGTCAGCAGCCGCGGTAAGACGTAGGGTGCAAGCGTTGTCCGGAATTAC1170.1888162672476398No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1160.18720245299765997No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1120.18074719599774067No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1070.17267812474784153No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT980.15815379649802308No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT940.15169853949810377No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT900.14524328249818447No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT890.14362946824820463No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC850.1371742112482853No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT840.1355603969983055No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT780.12587751149842652No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT760.12264988299846688No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT720.11619462599854757No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT640.10328411199870896No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC620.1000564834987493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)