FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003935823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003935823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44948
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16233.610839191955148No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14883.310492124232446No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11162.4828690931743345No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10792.400551748687372No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10422.3182344042004095No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10162.260389783750111No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9972.218118714959509No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9932.20921954258254No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9502.113553439530124No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8311.8488030613152975No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8221.8287799234671176No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8081.797632820147726No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7461.6596956483047078No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6951.5462312004983536No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6941.5440064074041113No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6851.5239832695559312No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6671.4839369938595712No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6341.4105188217495772No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6091.3548989943935215No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6061.3482246151107946No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6021.3393254427338257No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5931.3193023048856456No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5571.2392097534929252No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5301.1791403399483849No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5001.1123965471211177No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4541.0100560647859749No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3490.7764527898905402No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3480.774227996796298No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3300.7341817210999377No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3030.6741123075553973No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2780.6184924801993414No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2740.6095933078223725No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2730.6073685147281304No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2650.5895701699741924No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2650.5895701699741924No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2380.529500756429652No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2350.5228263771469254No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2270.5050280323929874No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2140.4761057221678384No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2060.4583073774139005No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2020.4494082050369315No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1980.4405090326599626No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1940.4316098602829937No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1930.42938506718875147No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1840.40936192934057136No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1790.39823796386936017No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1770.39378837768087566No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1740.387113998398149No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1730.38488920530390675No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1650.36709086054996887No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1600.3559668950787577No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1510.33594375723057757No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.2825487229687639No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1250.2780991367802794No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1250.2780991367802794No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.27142475749755274No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1220.27142475749755274No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1140.25362641274361486No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1140.25362641274361486No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1110.24695203346088812No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1110.24695203346088812No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1060.23582806798967695No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1060.23582806798967695No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1000.22247930942422356No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA980.21802972323573905No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA980.21802972323573905No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA970.21580493014149685No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA970.21580493014149685No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA960.2135801370472546No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA950.2113553439530124No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA940.20913055085877014No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.20690575776452788No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA920.20468096467028568No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.20023137848180117No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA900.20023137848180117No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.19800658538755897No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA890.19800658538755897No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.19133220610483226No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA840.1868826199163478No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA840.1868826199163478No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.18465782682210555No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.18465782682210555No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA830.18465782682210555No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA780.17353386135089435No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.17353386135089435No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA770.17130906825665213No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA770.17130906825665213No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA760.1690842751624099No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA740.1646346889739254No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA730.16240989587968319No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA730.16240989587968319No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA710.15796030969119873No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA710.15796030969119873No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA700.15573551659695647No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA700.15573551659695647No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA700.15573551659695647No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA690.15351072350271425No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA690.15351072350271425No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA690.15351072350271425No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.15351072350271425No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA680.15128593040847202No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA680.15128593040847202No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.15128593040847202No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA670.1490611373142298No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA660.14683634421998754No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA650.1446115511257453No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA650.1446115511257453No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA640.14238675803150308No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA640.14238675803150308No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1379371718430186No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1379371718430186No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA620.1379371718430186No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA620.1379371718430186No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1379371718430186No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA610.13571237874877637No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA610.13571237874877637No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.13571237874877637No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA600.13348758565453414No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA590.13126279256029189No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA590.13126279256029189No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.12903799946604966No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.12458841327756519No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA560.12458841327756519No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA550.12236362018332296No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA540.12013882708908072No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.11791403399483848No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA520.11568924090059624No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA500.11123965471211178No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA480.1067900685236273No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA480.1067900685236273No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA470.10456527542938507No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA470.10456527542938507No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA460.10234048233514284No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA460.10234048233514284No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA450.10011568924090058No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA450.10011568924090058No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA450.10011568924090058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)