FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003935973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003935973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60189
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA17862.9673196098954957No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA17562.917476615328382No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17142.847696422934423No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16212.6931831397763712No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA8931.4836598049477479No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7981.3258236554852216No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7881.3092093239628504No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7711.2809649603748192No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7601.262689195700211No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7131.1846018375450664No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6841.1364202761301898No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6801.1297745435212414No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6721.1164830783033444No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6661.1065144793899218No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6581.0932230141720247No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6541.0865772815630763No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6521.083254415258602No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6411.0649786505839938No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6331.0516871853660967No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6251.0383957201482No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6211.0317499875392513No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6121.0167970891691172No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6111.0151356560168803No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6010.998521324494509No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5890.9785841266676636No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5460.9071425011214674No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4630.7692435494857864No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4260.707770522853013No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4250.7061090897007759No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4000.6645732608948479No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3980.6612503945903737No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3780.6280217315456312No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3690.6130688331754972No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA3680.6114074000232601No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3620.6014388011098374No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3560.5914702021964147No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3510.583163036435229No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3470.5765173038262805No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3470.5765173038262805No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3440.5715330043695692No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3380.5615644054561465No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3350.5565801059994351No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3340.554918672847198No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3310.5499343733904866No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3290.5466115070860124No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3210.5333200418681154No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3210.5333200418681154No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3180.528335742411404No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA3130.5200285766502184No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3110.5167057103457442No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3090.51338284404127No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3080.5117214108890329No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3050.5067371114323215No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3020.5017528119756102No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3000.4984299456711359No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2950.4901227799099503No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2910.4834770473010019No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2800.46520128262639354No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2700.4485869511040223No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2680.4452640847995481No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2610.4336340527338883No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2610.4336340527338883No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2190.3638538603399292No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2140.35554669457874366No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2050.3405937962086096No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1970.3273023309907126No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA1890.3140108657728156No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1840.30570370001163005No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA1830.3040422668593929No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1780.29573510109820733No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1730.2874279353370217No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1670.277459336423599No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1630.2708136038146505No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1600.26582930435793917No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1560.25918357174899065No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1540.25586070544451645No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1530.2541992722922793No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1470.2442306733788566No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1430.23758494076990813No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1420.23592350761767103No Hit
GGACTACGCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1380.2292777750087225No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1360.2259549087042483No Hit
GGACTACGAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1340.22263204239977405No Hit
GGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1340.22263204239977405No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1300.2159863097908256No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1300.2159863097908256No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1260.20934057718187707No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1250.20767914402963997No Hit
GGACTACAGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1250.20767914402963997No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA1240.20601771087740287No Hit
GGACTACGAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1220.2026948445729286No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1200.19937197826845438No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1150.19106481250726878No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1130.18774194620279455No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA1130.18774194620279455No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA1100.1827576467460832No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA1070.17777334728937183No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA1040.17278904783266047No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1030.17112761468042334No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1030.17112761468042334No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA1020.1694661815281862No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA980.16282044891923775No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA970.16115901576700062No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA970.16115901576700062No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA910.1511904168535779No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA840.13956038478791805No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA830.13789895163568094No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA800.13291465217896958No Hit
GGACTACCCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA800.13291465217896958No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA790.13125321902673245No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA790.13125321902673245No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.12959178587449532No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA780.12959178587449532No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA780.12959178587449532No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA770.12793035272225822No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.1262689195700211No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA760.1262689195700211No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA750.12460748641778398No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA730.12128462011330975No Hit
GGACTACCAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA730.12128462011330975No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA710.11796175380883551No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA710.11796175380883551No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA690.11463888750436126No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA690.11463888750436126No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA680.11297745435212415No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA670.11131602119988702No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA670.11131602119988702No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA670.11131602119988702No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA660.1096545880476499No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA660.1096545880476499No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA660.1096545880476499No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA650.1079931548954128No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA640.10633172174317566No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA640.10633172174317566No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA640.10633172174317566No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA630.10467028859093853No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA620.10300885543870143No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA620.10300885543870143No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA620.10300885543870143No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCA610.1013474222864643No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.1013474222864643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)