FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003935979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003935979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71869
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA31254.348189066217702No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA25013.479942673475351No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA21663.0138168055768135No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17802.4767284921176027No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17392.4196802515688267No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15552.1636588793499283No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14251.982774214195272No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13711.90763750713103No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13341.8561549485870126No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12461.733709944482322No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12441.7309271034799427No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12061.6780531244347352No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10921.5194311872991135No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10541.4665572082539065No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10261.427597434220596No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10161.4136832292086992No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9511.323240896631371No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9291.2926296456051982No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8451.1757503235052666No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7991.111744980450542No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7881.0964393549374556No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7651.0644366834100933No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7641.0630452629089036No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7251.0087798633625067No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7100.9879085558446619No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7040.9795600328375238No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7000.9739943508327652No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6170.8585064492340231No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5900.820938095701902No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5830.8111981521935744No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5810.808415311191195No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5810.808415311191195No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5790.8056324701888157No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5710.7945011061792984No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5570.7750212191626431No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5520.7680641166566948No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5260.7318871836257635No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4980.6929274095924529No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4710.655359056060332No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4640.6456191125520043No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4580.6372705895448664No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4570.6358791690436767No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4470.62196496403178No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4370.6080507590198834No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4250.5913537130056075No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4220.5871794515020384No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4170.5802223489960902No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4110.5718738259889522No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3970.5523939389722968No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3630.5050856419318482No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3550.49395427792233093No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3530.4911714369199516No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3390.4716915499032963No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3380.47030012940210664No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3350.4661258678985376No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3270.4549945038890203No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3110.4327317758699857No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3070.4271660938652271No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3020.42020899135927864No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3020.42020899135927864No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2970.4132518888533303No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2730.3798577968247784No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2680.3729006943188301No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2650.3687264328152611No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2590.3603779098081231No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA2560.35620364830455414No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2540.3534208073021748No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2450.3408980227914678No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2380.3311580792831401No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2270.31585245377005383No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2180.30332966925934685No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1970.2741098387343639No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1920.26715273622841557No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1890.2629784747248466No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1880.2615870542236569No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1820.25323853121651896No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1820.25323853121651896No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1800.2504556902141396No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1780.24767284921176028No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1720.2393243262046223No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1720.2393243262046223No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1600.22262728019034633No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1580.219844439187967No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1550.21567017768439803No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1540.21427875718320835No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1530.21288733668201865No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1410.19619029066774268No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1400.19479887016655303No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1320.18366750615703573No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1320.18366750615703573No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1310.18227608565584605No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1240.17253614214751842No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1230.17114472164632874No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1140.15862193713562175No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1130.15723051663443208No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1090.15166483462967342No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA1090.15166483462967342No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1050.14609915262491477No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1000.13914205011896646No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA990.1377506296177768No Hit
GGACTACGCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA970.13496778861539746No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.13357636811420778No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA960.13357636811420778No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA950.13218494761301813No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.13079352711182846No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA930.1294021066106388No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA920.12801068610944913No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA920.12801068610944913No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.11966216310231115No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.11548790159874216No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA820.1140964810975525No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA820.1140964810975525No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA810.11270506059636283No Hit
GGACTACGCGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA780.10853079909279384No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA780.10853079909279384No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA770.10713937859160418No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA770.10713937859160418No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA770.10713937859160418No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA760.1057479580904145No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA720.10018227608565584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)