FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71160
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA45396.3785834738617195No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA42025.905002810567734No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA18782.639123102866779No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA17422.4480044969083754No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA15662.2006745362563236No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA15652.1992692523889823No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA15442.1697582911748174No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA14822.082630691399663No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA14161.9898819561551433No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13901.9533445756042722No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13751.9322653175941542No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12351.7355255761663857No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12231.7186621697582911No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11321.5907813378302418No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9841.3827993254637436No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9191.2914558740865656No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8881.2478920741989883No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8291.164980326025857No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8171.1481169196177627No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8141.143901068015739No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7951.1172006745362564No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7931.1143901068015738No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7761.0905002810567734No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7020.9865092748735245No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6800.9555930297920181No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6720.9443507588532885No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5470.7686902754356381No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5250.7377740303541315No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4680.657672849915683No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4680.657672849915683No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4290.602866779089376No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4150.5831928049465992No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3750.5269814502529512No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3720.5227655986509274No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA3690.5185497470489039No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3660.5143338954468802No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3500.491849353569421No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA3300.463743676222597No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3260.45812254075323217No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3220.45250140528386734No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3080.4328274311410905No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2930.41174817313097245No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2870.40331646992692527No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2800.39347948285553686No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2450.3442945474985947No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2430.3414839797639123No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2420.3400786958965711No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.3218100056211355No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.3007307476110174No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2050.2880831928049466No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1980.2782462057335582No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1960.27543563799887577No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1940.27262507026419336No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1940.27262507026419336No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1930.2712197863968522No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1920.26981450252951095No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1920.26981450252951095No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.2613827993254637No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.25857223159078135No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1830.2571669477234401No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1820.25576166385609894No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1770.2487352445193929No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1660.2332771219786397No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1640.2304665542439573No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1610.22625070264193367No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA1580.22203485103991005No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1560.21922428330522764No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1550.21781899943788646No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1540.21641371557054526No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1440.2023608768971332No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1440.2023608768971332No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1430.200955593029792No Hit
GGACTACGCGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1420.19955030916245084No Hit
GGACTACGCGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1410.19814502529510963No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1350.1897133220910624No Hit
GGACTACGAGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1310.1840921866216976No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1300.1826869027543564No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1280.17987633501967398No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1270.17847105115233278No Hit
GGACTACGAGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1270.17847105115233278No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1260.17706576728499157No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1220.17144463181562675No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTCCCCTGGCTTTCGCGCCTCA1220.17144463181562675No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1200.16863406408094433No Hit
GGACTACTGGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1200.16863406408094433No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCATCA1180.16582349634626195No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1170.16441821247892074No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1160.16301292861157954No Hit
GGACTACCGGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1130.15879707700955592No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1100.15458122540753233No Hit
GGACTACAGGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA1090.15317594154019112No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1080.15177065767284992No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1080.15177065767284992No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1080.15177065767284992No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1070.1503653738055087No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1020.1433389544688027No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1010.1419336706014615No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1010.1419336706014615No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA960.13490725126475547No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA940.13209668353007306No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA940.13209668353007306No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA940.13209668353007306No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.12928611579539068No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCATCA920.12928611579539068No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.12928611579539068No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA900.12647554806070826No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA900.12647554806070826No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA900.12647554806070826No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA870.12225969645868466No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.11944912872400226No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA850.11944912872400226No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA840.11804384485666106No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA820.11523327712197864No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA820.11523327712197864No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA810.11382799325463744No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA810.11382799325463744No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA790.11101742551995503No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA780.10961214165261382No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA760.10680157391793142No Hit
GGACTACTCGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA760.10680157391793142No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.10680157391793142No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA760.10680157391793142No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA750.10539629005059022No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA750.10539629005059022No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA740.10399100618324901No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA730.1025857223159078No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA730.1025857223159078No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA730.1025857223159078No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTCCCCTAGCTTTCGCACTTTA720.1011804384485666No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA720.1011804384485666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)