FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58052
Sequences flagged as poor quality0
Sequence length239
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA22843.93440363811755No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA21033.622614207951492No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA12012.0688348377316887No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA11762.025769999310963No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA11692.013711844553159No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA11601.9982085027216978No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10641.832839523186109No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10331.7794391235444085No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10251.7656583752497763No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA9891.7036450079239305No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA9751.6795286984083235No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA8781.5124371253359057No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8081.3918555777578723No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA8001.37807482946324No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7851.3522359264108041No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA7841.350513332873975No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA7611.310893681526907No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7331.2626610624956935No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6871.1834217598015573No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6801.171363605043754No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6691.1524150761386343No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6661.1472472955281472No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6551.1282987666230277No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6541.1265761730861987No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5921.0197753738027975No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5891.0146075931923104No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5871.0111624061186522No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5590.9629297870874388No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA5490.9457038517191483No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA4960.8544063942672088No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4270.7355474402260043No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4260.7338248466891752No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4230.7286570660786881No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4100.7062633500999105No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4100.7062633500999105No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4020.692482601805278No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4000.68903741473162No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3960.6821470405843038No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3700.6373596086267485No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3650.6287466409426031No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3610.6218562667952869No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3590.6184110797216289No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3590.6184110797216289No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3530.6080755185006546No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3470.5977399572796802No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3340.5753462413009026No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3330.5736236477640736No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3300.5684558671535864No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3220.554675118858954No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3210.552952525322125No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3190.5495073382484669No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3010.518500654585544No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3000.5167780610487149No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2930.5047199062909116No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2920.5029973127540825No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2750.4737132226279887No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2710.4668228484806725No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2710.4668228484806725No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2700.46510025494384344No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2690.46337766140701436No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2670.4599324743333563No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2630.45304210018604013No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.4409839454282367No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2550.4392613518914077No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2520.4340935712809206No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2500.4306483842072624No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2470.4254806035967753No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.42375801005994623No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2250.3875835457865362No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2210.38069317163922No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2130.3669124233445876No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2110.36346723627092953No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2090.36002204919727143No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2090.36002204919727143No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2080.35829945566044236No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.35485426858678426No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.35485426858678426No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2040.35140908151312616No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2010.346241300902639No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2000.34451870736581No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1980.3410735202921519No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1910.3290153655343485No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1900.32729277199751944No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1890.3255701784606904No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1890.3255701784606904No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1880.32384758492386134No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1800.31006683662922896No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1780.30662164955557086No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1730.29800868187142565No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1700.2928409012609385No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1690.2911183077241094No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1670.2876731206504513No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1610.277337559429477No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1550.2670019982085027No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1540.26527940467167366No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1540.26527940467167366No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1530.26355681113484464No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1510.26011162406118654No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1500.25838903052435747No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1390.2394405016192379No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1240.2136015985668022No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1030.17742713429339213No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1020.17570454075656308No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1020.17570454075656308No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA1010.17398194721973403No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1000.172259353682905No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA990.17053676014607594No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA930.16020119892510162No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA910.15675601185144356No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA880.15158823124095638No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA870.14986563770412734No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA840.1446978570936402No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA810.13953007648315302No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA800.13780748294632397No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCA780.13436229587266588No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA750.12919451526217873No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA750.12919451526217873No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA730.12574932818852064No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA730.12574932818852064No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA730.12574932818852064No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA730.12574932818852064No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA720.12402673465169159No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCA720.12402673465169159No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA700.1205815475780335No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA690.11885895404120445No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA680.1171363605043754No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA660.11369117343071727No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA660.11369117343071727No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA660.11369117343071727No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA650.11196857989388823No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA630.10852339282023014No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA610.10507820574657205No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA600.103355612209743No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA590.10163301867291395No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA590.10163301867291395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)