FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65991
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA14102.136654998408874No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA12771.9351123638071857No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12721.9275355730326862No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA10171.541119243533209No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9721.4729281265627132No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA9201.3941295025079177No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8941.35473019048052No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA8441.2789622827355245No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8271.253201194102226No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7871.1925868679062297No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7661.1607643466533315No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7661.1607643466533315No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7651.1592489884984316No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7231.0956039459926352No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6921.048627843190738No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6601.0001363822339409No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6590.9986210240790411No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6520.9880135169947417No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6490.9834674425300419No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6350.9622524283614433No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6250.9470988468124442No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6240.9455834886575442No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6120.9273991907987453No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5910.895576669545847No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5850.8864845206164478No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5840.8849691624615477No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5710.8652695064478488No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5620.8516312830537498No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5540.8395084178145504No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5470.8289009107302511No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5460.8273855525753512No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5430.8228394781106515No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5420.8213241199557516No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5420.8213241199557516No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5220.7910169568577534No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5210.7895015987028533No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5180.7849555242381536No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5090.7713173008440545No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5030.762225151914655No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5000.7576790774499553No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4900.7425254959009562No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4840.7334333469715567No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4700.712218332802958No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4640.7031261838735585No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4610.6985801094088587No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4590.6955493930990589No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4590.6955493930990589No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4350.6591807973814612No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4260.6455425739873619No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4080.6182661271991635No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4040.6122046945795638No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3830.5803821733266658No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3790.5743207407070661No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3640.5515903683835675No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3580.542498219454168No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3470.525829279750269No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3450.5227985634404692No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3370.5106756982012699No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3340.5061296237365701No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3330.5046142655816702No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3320.5030989074267703No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3310.5015835492718704No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2920.44248458123077383No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2890.4379385067660742No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2870.4349077904562743No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2820.42733099968177474No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.41520813444257554No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.41520813444257554No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2690.4076313436680759No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2610.3955084784288767No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2610.3955084784288767No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2530.3833856131896774No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2490.37732418057007777No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.3621705990210786No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2290.3470170174720795No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA2240.33944022669758No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2160.3273173614583807No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2140.3242866451485809No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2100.3182252125289812No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1900.28791804943098304No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1830.2773105423466836No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1760.26670303526238426No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1700.2576108863329848No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1690.2560955281780849No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1650.2500340955584852No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1610.24397266293888561No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1600.2424573047839857No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1590.24094194662908577No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1570.237911230319286No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1500.22730372323498657No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1500.22730372323498657No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1450.21972693246048702No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1420.21518085799578732No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1350.20457335091148793No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1330.20154263460168811No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1300.19699656013698838No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1300.19699656013698838No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1280.19396584382718854No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1260.19093512751738873No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1230.186389053052689No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1210.18335833674288918No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1210.18335833674288918No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1190.18032762043308936No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1170.17729690412328955No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1140.1727508296585898No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1120.16972011334879No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1110.16820475519389008No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1110.16820475519389008No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1100.16668939703899016No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1080.16365868072919035No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1080.16365868072919035No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1070.16214332257429045No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1050.1591126062644906No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1050.1591126062644906No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1040.1575972481095907No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1040.1575972481095907No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1040.1575972481095907No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.1575972481095907No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.1575972481095907No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1030.1560818899546908No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA1030.1560818899546908No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1000.15153581548999107No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA990.15002045733509115No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA980.14850509918019125No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA970.14698974102529133No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA970.14698974102529133No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA970.14698974102529133No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA940.1424436665605916No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA940.1424436665605916No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA940.1424436665605916No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA940.1424436665605916No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGA930.1409283084056917No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA920.13941295025079176No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA910.13789759209589186No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA890.13486687578609205No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.13486687578609205No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA890.13486687578609205No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA880.13335151763119213No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA870.1318361594762922No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.13032080132139232No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA840.1272900850115925No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA830.12577472685669258No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA820.12425936870179266No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA790.11971329423709294No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.11971329423709294No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.11819793608219302No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA760.11516721977239322No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA750.11365186161749329No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA730.11062114530769346No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA730.11062114530769346No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA730.11062114530769346No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA710.10759042899789366No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA700.10607507084299374No Hit
GGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGA680.10304435453319391No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA660.10001363822339408No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGA660.10001363822339408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)