FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62456
Sequences flagged as poor quality0
Sequence length239
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT45187.233892660432945No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT35935.7528500064045085No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT33765.405405405405405No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT29724.758550019213526No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC21933.5112719354425517No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC18262.92365825541181No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT18112.899641347508646No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC16302.6098373254771356No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC14592.336044575381068No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT14212.2752017420263866No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT14092.2559882157038555No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC13332.134302548994492No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT11491.8396951453823491No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC9641.543486614576662No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC9211.474638145254259No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC9071.4522223645446395No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC7981.2776995004483156No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT6951.1127833995132574No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6711.0743563468681951No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6381.0215191494812348No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6331.0135135135135136No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC5840.9350582810298449No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC5630.9014346099654156No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT5510.8822210836428845No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC5340.8550019213526322No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC5100.81657486870757No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT4790.7669399257076982No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATGAC4660.7461252721916228No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4530.7253106186755476No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT4480.7173049827078264No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC4480.7173049827078264No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC4300.6884846932240297No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC4090.6548610221596004No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3990.6388497502241578No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3900.6244396054822595No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC3780.6052260791597285No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT3510.5619956449340335No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3510.5619956449340335No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3470.5555911361598566No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3110.4979505571922634No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATGAC3090.49474830280517484No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATGAC3090.49474830280517484No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATGAC3090.49474830280517484No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3030.48514153964390927No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC2970.47553477648264375No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2870.4595235045472012No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2730.4371077238375817No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT2650.42429870628922756No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT2470.39547841680543105No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2270.3634558729345459No Hit
GTGCCAGCCGCCGCGGTAATACGGAGACTACGAGCGTTACTCGGATTCAC2160.34584347380555913No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT2120.3394389650313821No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT2090.3346355834507493No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT2020.32342769309593955No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1980.3170231843217625No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1960.313820929934674No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1930.3090175483540412No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT1890.30261303957986424No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1810.2898040220315102No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1770.2833995132573332No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1760.28179838606378893No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1720.27539387728961184No Hit
GTGTCAGCCGCCGCGGTAATACGGAGACTACGAGCGTTACTCGGATTCAC1660.2657871141283464No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1600.2561803509670808No Hit
GTGCCAGCAGCCGCGGTAATACGGAGACTACGAGCGTTACTCGGATTCAC1500.24016907903163828No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1490.238567951838094No Hit
GTGTCAGCAGCCGCGGTAATACGGAGACTACGAGCGTTACTCGGATTCAC1480.23696682464454977No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1410.22575893428973998No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1380.2209555527091072No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1290.2065454079672089No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1250.20014089919303188No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1230.1969386448059434No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1140.18252850006404508No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1120.1793262456769566No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGAATGAC1120.1793262456769566No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1120.1793262456769566No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1080.17292173690277957No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1070.1713206097092353No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1050.1681183553221468No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1020.16331497374151402No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1020.16331497374151402No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC950.15210708338670426No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGATTCAT940.15050595619315998No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC910.14570257461252722No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC880.14089919303189447No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGAATGAC870.1392980658383502No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC870.1392980658383502No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT850.13609581145126168No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGAATGAC800.1280901754835404No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC790.12648904828999616No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGAATGAC780.12488792109645191No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT760.12168566670936339No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT740.11848341232227488No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAC680.10887664916100935No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC640.10247214038683232No Hit
GTGCCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT640.10247214038683232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)