FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56357
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA22483.9888567524885996No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA21143.751086821512856No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA14092.5001330801852477No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA13642.420284969036677No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11652.0671788775129976No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11251.9962027787142682No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9531.6910055538797308No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9191.630675869900811No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9111.616480650141065No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9051.6058342353212556No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8391.488723672303352No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7951.4106499636247494No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7931.4071011586848128No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7931.4071011586848128No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7871.3964547438650035No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7621.3520946821157975No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5881.043348652341324No Hit
GGACTACGCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5791.02737903011161No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5751.020281420231737No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5741.0185070177617688No Hit
GGACTACGAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5270.9351101016732616No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5230.9280124917933886No Hit
GGACTACGCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5110.9067196621537698No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5060.8978476498039286No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5040.8942988448639921No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5020.8907500399240555No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4990.885426832514151No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4920.8730060152243732No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4900.8694572102844368No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4880.8659084053445003No Hit
GGACTACGAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4870.8641340028745319No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4740.841066770764945No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4680.8304203559451354No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4620.819773941125326No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4430.7860602941959295No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4260.7558954522064695No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4240.752346647266533No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4150.7363770250368188No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4130.7328282200968823No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4110.7292794151569459No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3940.6991145731674858No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3910.6937913657575812No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3810.6760473410578988No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3720.6600777188281847No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3620.6423336941285022No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3580.6352360842486293No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3410.6050712422591692No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3410.6050712422591692No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3330.5908760224994233No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3200.5678087903898362No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2900.514576716290789No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2740.4861862767712973No Hit
GGACTACCCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2670.4737654594815196No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2610.4631190446617102No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2500.44360061749205953No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2470.4382774100821548No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2440.43295420267225015No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2410.4276309952623454No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2380.4223077878524407No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2370.4205333853824725No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2350.416984580442536No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2350.416984580442536No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2340.4152101779725677No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2320.41166137303263123No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2310.409886970562663No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2230.3956917508029172No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2140.379722128573203No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2090.3708501162233618No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2070.3673013112834253No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA2060.36552690881345706No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2040.3619781038735206No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2030.36020370140355235No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2010.35665489646361587No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1990.3531060915236794No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1990.3531060915236794No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1960.34778288411377467No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA1950.34600848164380643No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1940.34423407917383825No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1860.33003885941409233No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1760.3122948347144099No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1570.2785811877850134No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1480.26261156555529924No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1450.2572883581453945No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1410.2501907482655216No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1360.2413187359156804No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1360.2413187359156804No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1350.23954433344571213No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1280.22712351615593449No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA1260.22357471121599803No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1220.21647710133612508No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1200.21292829639618857No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1170.20760508898628385No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1130.20050747910641092No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA1130.20050747910641092No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1120.19873307663644266No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.1951842716965062No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1030.18276345440672853No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1020.1809890519367603No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA990.17566584452685557No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA970.17211703958691912No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA920.1632450272370779No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA920.1632450272370779No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA900.15969622229714142No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.15614741735720494No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA880.15614741735720494No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.1525986124172685No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA840.149049807477332No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA840.149049807477332No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA800.14195219759745906No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA790.14017779512749082No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA790.14017779512749082No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.13840339265752258No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.13840339265752258No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.13840339265752258No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA760.1348545877175861No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA750.13308018524761786No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA740.13130578277764962No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA710.1259825753677449No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA700.12420817289777668No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA700.12420817289777668No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA690.12243377042780845No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA690.12243377042780845No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA680.1206593679578402No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA680.1206593679578402No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA670.11888496548787197No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA660.11711056301790372No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.11711056301790372No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA640.11356175807796724No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA630.11178735560799902No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA630.11178735560799902No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.10823855066806254No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA600.10646414819809429No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA590.10468974572812606No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA580.10291534325815781No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA580.10291534325815781No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA580.10291534325815781No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA570.10114094078818958No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA570.10114094078818958No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA570.10114094078818958No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA570.10114094078818958No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA570.10114094078818958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)