FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49591
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA18983.827307374321954No Hit
GGACTACGGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA18343.6982516988969776No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15513.12758363412716No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15043.0328083724869432No Hit
GGACTACGCGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA10332.0830392611562583No Hit
GGACTACGCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA9691.953983585731282No Hit
GGACTACCGGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA9231.8612248190195801No Hit
GGACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA9141.843076364662943No Hit
GGACTACGAGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA8951.804762961021153No Hit
GGACTACAGGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA8141.6414268718114176No Hit
GGACTACGAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA8121.6373938819543867No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7901.5930309935270515No Hit
GGACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA7831.5789155290274446No Hit
GGACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA7801.572866044241899No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7721.5567340848137767No Hit
GGACTACTGGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA7561.5244701659575326No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7431.4982557318868344No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7101.431711399245831No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7051.4216289246032547No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7011.4135629448891935No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6501.3107217035349157No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6451.3006392288923394No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5981.2058639672521223No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5881.18569901796697No Hit
GGACTACCAGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA5581.125204170111512No Hit
GGACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA5101.02841241354278No Hit
GGACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA5061.020346433828719No Hit
GGACTACAAGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA5041.0163134439716883No Hit
GGACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4890.9860660200439596No Hit
GGACTACCCGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4880.9840495251154444No Hit
GGACTACACGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4850.9780000403298985No Hit
GGACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4450.8973402431892884No Hit
GGACTACTCGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4380.8832247786896816No Hit
GGACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4350.8771752939041357No Hit
GGACTACTAGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4350.8771752939041357No Hit
GGACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA4210.8489443649049223No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4080.822729930834224No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4070.8207134359057088No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3990.8045814764775866No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3730.75215260833619No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3650.736020648908068No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3630.7319876590510375No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3550.7158556996229155No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3500.7057732249803392No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3450.6956907503377628No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3450.6956907503377628No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3440.6936742554092477No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3440.6936742554092477No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3050.6150309531971527No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2730.5505031154846646No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2390.4819422879151459No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA2250.45371135891593234No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1760.3549031074186848No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1590.32062269363392554No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.30449073420580347No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1450.29239176463471195No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.2903752697061967No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.2802927950636204No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1380.2782763001351052No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.27625980520658994No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1370.27625980520658994No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.2681938254925289No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1310.2641608356354984No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.26012784577846787No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.2581113508499526No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1190.23996289649331534No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.22383093706519328No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1090.21979794720816279No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1060.21374846242261702No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.20971547256558648No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1000.2016494928515255No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA980.19761650299449496No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA940.18955052328043395No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA920.18551753342340344No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA900.18148454356637292No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.18148454356637292No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.17946804863785767No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.1774515537093424No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.17140206892379664No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA810.16333608920973564No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.15728660442418987No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.15728660442418987No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA770.1552701094956746No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA770.1552701094956746No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA770.1552701094956746No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.1512371196386441No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA740.14922062471012884No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.1472041297816136No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA730.1472041297816136No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.14518763485309835No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA710.14317113992458308No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA710.14317113992458308No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.14317113992458308No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.1391381500675526No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.13712165513903732No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA670.13510516021052207No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA670.13510516021052207No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA670.13510516021052207No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.1330886652820068No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA660.1330886652820068No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.1330886652820068No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA640.1290556754249763No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA620.1250226855679458No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA620.1250226855679458No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1250226855679458No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.1250226855679458No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA620.1250226855679458No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.1250226855679458No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA620.1250226855679458No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.12300619063943055No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA610.12300619063943055No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.12300619063943055No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA610.12300619063943055No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA610.12300619063943055No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.11897320078240003No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA590.11897320078240003No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA580.11695670585388479No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.11695670585388479No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA580.11695670585388479No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA570.11494021092536952No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA570.11494021092536952No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.11292371599685427No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.11090722106833903No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.11090722106833903No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.11090722106833903No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA540.10889072613982376No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA540.10889072613982376No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA530.10687423121130851No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.10687423121130851No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA520.10485773628279324No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA510.102841241354278No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.10082474642576275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)