FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003936974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003936974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41243
Sequences flagged as poor quality0
Sequence length239
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC19984.844458453555755No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC17134.153432097568072No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC17024.126760904880828No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC16854.085541788909633No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC16263.9424872099507797No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC14373.4842276265063163No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC13813.3484470091894383No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC13293.222365007395194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT11232.722886307979536No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT9802.3761608030453654No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT9742.361612879761414No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT9452.2912979172223165No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT7451.8063671410906095No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC7041.7069563319836094No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC6271.5202579831729022No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT6081.47418955944039No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT6021.4596416361564386No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT5891.4281211357078776No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5801.406299250781951No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5611.3602308270494388No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4551.103217515699634No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC4321.0474504764444876No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4321.0474504764444876No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4181.0135053221152681No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC4181.0135053221152681No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4100.9941080910699998No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC4050.9819848216667071No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4030.9771355139053899No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC4000.9698615522634143No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3990.9674368983827558No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3870.9383410518148535No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3800.9213684746502436No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3350.8122590500206096No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2980.7225468564362437No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2980.7225468564362437No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2760.669204471061756No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2050.4970540455349999No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2010.4873554300123657No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1830.4437116601605121No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1730.4194651213539268No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1560.3782460053827316No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1530.370972043740756No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1530.370972043740756No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1470.35642412045680477No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1460.3539994665761463No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1450.35157481269548774No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1440.3491501588148292No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1360.3297529277695609No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1350.32732827388890234No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1350.32732827388890234No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1250.303081735082317No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1250.303081735082317No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1180.28610915791770725No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1050.2545886574691463No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC980.23761608030453654No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT950.2303421186625609No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT950.2303421186625609No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT930.22549281090124387No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT920.2230681570205853No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT920.2230681570205853No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT910.22064350313992678No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC900.2182188492592682No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC870.21094488761729263No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT830.2012462720946585No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT820.19882161821399993No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT810.19639696433334142No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT810.19639696433334142No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT800.19397231045268287No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT760.18427369493004875No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC760.18427369493004875No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT750.1818490410493902No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT720.1745750794074146No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGAATTAT710.17215042552675605No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGAATTAT710.17215042552675605No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT690.167301117765439No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT680.16487646388478044No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT670.16245181000412193No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT650.15760250224280484No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC630.15275319448148778No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC620.15032854060082923No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC600.14547923283951214No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC600.14547923283951214No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC560.13578061731687802No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTTCGGAATTAT560.13578061731687802No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT550.13335596343621947No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT530.1285066556749024No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC520.12608200179424386No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT510.12365734791358533No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC510.12365734791358533No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC500.12123269403292679No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC490.11880804015226827No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC490.11880804015226827No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT480.11638338627160973No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT470.1139587323909512No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT470.1139587323909512No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC470.1139587323909512No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT460.11153407851029265No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT440.10668477074897559No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT440.10668477074897559No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT430.10426011686831704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)