FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003937072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109012
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC69136.341503687667413No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC63805.852566689905698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC59485.456280042564121No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC59205.430594796903093No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC31632.9015154294940007No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC28862.647414963490258No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC27872.55659927347448No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC27312.5052287821524235No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC26412.4226690639562616No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC25782.3648772612189486No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC24172.2171870986680364No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC23712.174989909367776No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT11311.037500458665101No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT10240.9393461270318864No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC10120.9283381646057316No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT10010.9182475323817562No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC9860.9044875793490624No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC9710.8907276263163689No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC9630.8833889846989321No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT9600.8806369940923936No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT9580.8788023336880344No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC9120.8366051443877738No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT8620.790738634278795No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8580.7870693134700768No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT8540.7833999926613584No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC8380.7687227094264851No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT8370.7678053792243056No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC8180.7503761053828937No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8140.7467067845741754No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT8030.7366161523502No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT8010.7347814919458409No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC7960.7301948409349429No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT7720.7081789160826332No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT7680.7045095952739149No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC7360.6751550288041683No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC7330.6724030381976296No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT7090.6503871133453197No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC6540.5999339522254431No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6410.5880086595971086No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6250.5733313763622353No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6060.5559021025208234No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC5740.526547536051077No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC5680.5210435548379995No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC5680.5210435548379995No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC5570.5109529226140241No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC5260.4825156863464573No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC5240.4806810259420981No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC5220.4788463655377389No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC5050.4632517521006862No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC4860.4458224782592742No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC4840.4439878178549151No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC4730.4338971856309397No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC4580.4201372325982461No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC4280.3926173265328588No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC4220.3871133453197813No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC3930.36051076945657357No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3750.3439988258173412No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC3750.3439988258173412No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3710.3403295050086229No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC3610.33115620298682713No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3580.32840421238028844No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3500.3210655707628518No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC3480.31923091035849266No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC3190.2926283344952849No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3110.28528969287784833No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC3020.2770337210582321No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT3000.27519906065387295No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2950.27061240964297506No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT2940.2696950794407955No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2940.2696950794407955No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC2930.2687777492386159No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2820.2586871170146406No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC2760.25318313580156315No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT2730.2504311451950244No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC2670.24492716398194694No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT2610.23942318276886945No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC2470.2265805599383554No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC2310.2119032767034822No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2230.20456463508604555No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC2010.1843833706380949No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTCAT2010.1843833706380949No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1910.17521006861629912No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAT1840.16878875720104208No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT1830.1678714269988625No Hit
GTGTCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTCAT1810.16603676659450337No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT1800.1651194363923238No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1790.16420210619014422No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1690.15502880416834844No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT1670.1531941437639893No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1660.15227681356180972No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAT1640.15044215315745055No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAT1630.14952482295527098No Hit
GTGTCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTCAT1600.14677283234873223No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAT1580.14493817194437308No Hit
GTGCCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTCAT1580.14493817194437308No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1580.14493817194437308No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT1490.1366822001247569No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCGGAATCAT1470.13484753972039776No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1450.13301287931603858No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1440.132095549113859No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1400.12842622830514072No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1360.1247569074964224No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1320.1210875866877041No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1310.12017025648552453No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT1310.12017025648552453No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT1300.11925292628334495No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1300.11925292628334495No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1290.11833559608116538No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1260.11558360547462665No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1250.11466627527244708No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1250.11466627527244708No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1240.1137489450702675No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT1240.1137489450702675No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCGGAATCAT1220.11191428466590835No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1210.11099695446372877No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC1190.1091622940593696No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1180.10824496385719003No Hit
GTGTCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1150.10549297325065131No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1130.10365831284629215No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCGGAATCAT1100.10090632223975342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)