FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53004
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15322.8903478982718287No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13772.5979171383291826No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9341.7621311599124594No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8741.6489321560636934No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8511.605539204588333No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8461.5961059542676026No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7981.5055467511885896No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7751.4621537997132292No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7731.458380499584937No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7451.4055542977888462No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7111.3414081956078787No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6921.3055618443891028No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6611.2470756924005737No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA6351.198022790732775No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6001.1319900384876613No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5481.033884235152064No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5300.9999245339974341No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5210.9829446834201193No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA5160.9735114330993888No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5150.9716247830352427No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4820.9093653309184213No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4770.8999320805976907No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4750.8961587804693985No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4670.8810655799562297No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4620.8716323296354992No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4470.8433325786733077No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4460.8414459286091617No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4330.8169194777752622No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4300.8112595275828239No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4290.8093728775186779No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4250.8018262772620934No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4160.7848464266847784No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4150.7829597766206324No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4090.7716398762357558No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3810.7188136744396649No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3760.7093804241189344No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3750.7074937740547883No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3740.7056071239906423No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3700.6980605237340578No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3520.6641008225794279No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3450.6508942721304052No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3450.6508942721304052No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3430.647120972002113No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3340.6301411214247982No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3290.6207078711040677No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3160.5961814202701683No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3140.592408120141876No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3090.5829748698211455No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3030.5716549694362689No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2990.5641083691796845No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2950.5565617689231002No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2850.5376952682816392No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2800.5282620179609087No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2790.5263753678967624No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2790.5263753678967624No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2790.5263753678967624No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2730.5150554675118859No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2660.5018489170628632No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2550.4810957663572561No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2520.4754358161648178No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2520.4754358161648178No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2420.4565693155233567No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2340.44147611501018796No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2250.42449626443287297No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2200.4150630141121425No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2170.4094030639197042No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2160.4075164138555581No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2120.39996981359897366No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2060.38864991321409703No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2060.38864991321409703No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2010.3792166628933666No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2010.3792166628933666No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2000.37733001282922046No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1970.3716700626367821No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1930.3641234623801977No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1930.3641234623801977No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1910.3603501622519055No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1900.3584635121877594No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1870.35280356199532115No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1860.350916911931175No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1830.3452569617387367No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1810.34148366161044447No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1800.3395970115462984No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1790.3377103614821523No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1760.33205041128971396No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1760.33205041128971396No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1730.3263904610972757No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1730.3263904610972757No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1680.31695721077654515No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1650.31129726058410684No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1620.3056373103916685No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1610.30375066032752246No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1610.30375066032752246No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1600.30186401026337634No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1520.2867708097502075No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1500.28299750962191533No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1430.2697909591728926No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1360.2565844087238699No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1360.2565844087238699No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1320.2490378084672855No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1300.24526450833899327No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1270.23960455814655496No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.23960455814655496No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1240.23394460795411667No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1230.23205795788997058No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1230.23205795788997058No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1200.22639800769753224No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1170.22073805750509398No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1130.21319145724850952No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1110.20941815712021733No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1090.20564485699192514No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1040.1962116066711946No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1040.1962116066711946No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1040.1962116066711946No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1040.1962116066711946No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1030.19432495660704852No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1020.19243830654290242No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1020.19243830654290242No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1020.19243830654290242No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1010.19055165647875633No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1000.18866500641461023No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA990.1867783563504641No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA940.1773451060297336No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA900.1697985057731492No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA890.16791185570900308No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA840.15847860538827258No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.15281865519583426No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA780.14715870500339598No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA780.14715870500339598No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA780.14715870500339598No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA760.14338540487510376No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA730.13772545468266545No Hit
GGACTACGGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA720.13583880461851935No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA680.12829220436193495No Hit
GGACTACGGGGGTTTCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA620.11697230397705834No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA610.11508565391291224No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.11508565391291224No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA610.11508565391291224No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA600.11319900384876612No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA600.11319900384876612No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA590.11131235378462002No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA580.10942570372047393No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA580.10942570372047393No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA570.10753905365632782No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA570.10753905365632782No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA560.10565240359218173No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.10376575352803563No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.10376575352803563No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA550.10376575352803563No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA550.10376575352803563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)