FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85327
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA29773.4889308190842288No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA28403.3283720276114246No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA20042.34861181103285No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA18262.140002578316359No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17492.049761505736754No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17332.0310101140319006No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17212.016946570253261No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16561.9407690414522953No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16241.903266258042589No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15251.7872420218688105No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15071.766146706200851No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15041.7626308202561909No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15041.7626308202561909No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14641.7157523409940583No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13361.5657412073552333No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13131.5387860817795072No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12761.4954234884620343No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12551.4708122868494145No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12351.4473730472183481No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12171.4262777315503885No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12141.4227618456057285No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11511.3489282407678695No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11441.3407245068969964No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11411.3372086209523362No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11371.332520773026123No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11231.3161133052843765No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11201.3125974193397167No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10741.258687168188264No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10731.2575152062067105No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10561.2375918525203042No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10221.1977451451474914No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10121.1860255253319583No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10111.184853563350405No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9911.1614143237193386No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9891.159070399756232No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9781.1461788179591452No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9771.145006855977592No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8791.030154581785367No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8480.993823760357214No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8220.9633527488368278No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8190.9598368628921679No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7820.916474269574695No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7610.8918630679620753No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7570.887175220035862No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7540.8836593340912021No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7420.8695957903125622No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7180.8414687027552827No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA6780.7945902234931499No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA6290.7371640863970373No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6260.7336482004523773No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA4240.496911880178607No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA4120.4828483363999672No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3870.4535492868611342No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3830.4488614389349209No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3770.44182966704560106No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3770.44182966704560106No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3720.4359698571378344No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3700.43362593317472786No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3670.4301100472300678No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3630.4254221993038546No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3570.41839042741453464No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3520.41253061750676806No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3250.3808876440048285No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA3080.3609642903184221No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2910.3410409366320157No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2820.3304932787980358No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2740.32111758294560927No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2730.31994562096405593No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2700.316429735019396No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2680.31408581105628935No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2400.28127087557279645No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2310.2707232177388166No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2300.26955125575726324No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2290.26837929377570996No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2180.2554877119786234No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2160.25314378801551674No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2160.25314378801551674No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2140.2507998640524101No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2090.24494005414464354No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2090.24494005414464354No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1960.22970454838445042No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1910.22384473847668385No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1890.2215008145135772No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1790.20978119469804402No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1780.20860923271649065No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1680.19688961290095752No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1660.19454568893785085No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1540.18048214515921104No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1530.1793101831776577No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1500.17579429723299775No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1440.16876252534367786No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1420.1664186013805712No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1410.1652466393990179No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1390.16290271543591126No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1380.16173075345435795No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1380.16173075345435795No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1370.16055879147280464No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1270.14883917165727142No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1230.14415132373105818No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1180.13829151382329158No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1170.13711955184173827No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1160.13594758986018493No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1120.13125974193397166No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1090.1277438559893117No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1060.12422797004465175No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1040.12188404608154513No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA1010.11836816013688517No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA980.11485227419222521No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA980.11485227419222521No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA980.11485227419222521No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA950.11133638824756525No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA890.10430461635824533No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.10313265437669202No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA880.10313265437669202No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.1007887304135854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)