FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50221
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA24194.816710141176002No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA22674.514047908245555No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17563.4965452699070108No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17473.4786244797992874No Hit
GGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA10041.9991636964616393No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA9671.9254893371298858No Hit
GGACTACGAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA8761.7442902371517892No Hit
GGACTACGCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA8761.7442902371517892No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA8401.6726070767208938No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA7871.5670735349754088No Hit
GGACTACGAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA7541.5013639712470879No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA7451.483443181139364No Hit
GGACTACAGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA7381.4695047888333566No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6921.377909639393879No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA6821.357997650385297No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6701.3341032635749985No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6581.3102088767647002No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6521.2982616833595508No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6271.2484817108380957No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6241.242508114135521No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6241.242508114135521No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6031.2006929372174986No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5921.1787897493080584No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5901.174807351506342No Hit
GGACTACCAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA4460.8880747097827602No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA4310.8582067262698871No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA4080.8124091515501484No Hit
GGACTACCCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA4060.808426753748432No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3830.7626291790286932No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3790.7546643834252603No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3760.7486907867226857No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3640.7247963999123873No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3570.7108580076063798No Hit
GGACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3480.692937217498656No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3450.6869636207960813No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3420.6809900240935067No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA3350.6670516317874993No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3320.6610780350849247No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3250.6471396427789172No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3230.6431572449772007No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3170.6312100515720515No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTACTTCA3160.6292188526711934No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3110.6192628581669023No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2950.587403675753171No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2830.5635092889428724No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2830.5635092889428724No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2800.5575356922402979No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2630.5236853109257084No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2600.5177117142231337No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2540.5057645208179845No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.48983492961111885No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA2110.4201429680810816No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2090.4161605702793652No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA1960.3902749845682085No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA1900.3783277911630593No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1780.3544334043527608No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1650.3285478186416041No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1610.32058302303817127No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1520.3026622329304474No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1450.28872384062444No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1390.27677664721929074No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1390.27677664721929074No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA1290.2568646582107087No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1270.25288226040899225No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1260.25089106150813406No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1250.24889986260727584No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1170.23297027140041018No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA1150.22898787359869377No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1110.22102307799526094No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1040.2070846856892535No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1020.2031022878875371No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1020.2031022878875371No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1020.2031022878875371No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA980.19513749228410426No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.19115509448238785No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.18717269668067144No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.18717269668067144No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.1792079010772386No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.17124310547380578No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.16925190657294756No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA850.16925190657294756No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.16526950877123117No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA810.16128711096951473No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA790.15730471316779834No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.15531351426694012No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA780.15531351426694012No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA770.1533223153660819No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.1513311164652237No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA750.14933991756436552No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTCA720.14336632086179088No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA720.14336632086179088No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA710.1413751219609327No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA690.13739272415921625No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA690.13739272415921625No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA690.13739272415921625No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA690.13739272415921625No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA690.13739272415921625No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACTTCA680.13540152525835805No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA680.13540152525835805No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA670.13341032635749986No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA670.13341032635749986No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA650.12942792855578342No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA640.12743672965492525No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA640.12743672965492525No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA640.12743672965492525No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA630.12544553075406703No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCA630.12544553075406703No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA610.1214631329523506No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA600.11947193405149241No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA600.11947193405149241No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA600.11947193405149241No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA580.11548953624977598No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA580.11548953624977598No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA580.11548953624977598No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA560.11150713844805958No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA550.10951593954720137No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA550.10951593954720137No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA550.10951593954720137No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA540.10752474064634315No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA540.10752474064634315No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA520.10354234284462675No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA520.10354234284462675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)