FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47692
Sequences flagged as poor quality0
Sequence length239
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC29686.223265956554559No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC27615.78923089826386No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC19254.036316363331376No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC19043.992283821185943No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC12942.713243311247169No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC12482.6167910760714586No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC10112.1198523861444265No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC9201.9290447035142162No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8681.8200117420112387No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8041.685817327853728No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT7591.5914618803992284No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT7281.5264614610416842No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6821.4300092258659733No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6811.4279124381447623No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT6731.4111381363750735No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6221.3042019625933072No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6081.2748469344963516No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6001.2580726327266627No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5901.2371047555145518No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5651.1846850624842742No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5631.180491487041852No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5541.1616203975509518No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT5131.0756521009812967No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4871.0211356202298079No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT4540.9519416254298415No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC4520.9477480499874192No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4500.9435544745449972No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4470.9372641113813638No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT4450.9330705359389415No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC4180.8764572674662418No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT4180.8764572674662418No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4040.8471022393692863No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3820.8009729095026419No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3740.784198607732953No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC3600.7548435796359976No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3470.7275853392602533No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT3070.6437138304118091No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC2930.6143588023148536No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2850.5975845005451649No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC2820.5912941373815315No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT2630.5514551706785206No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2550.5346808689088317No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2550.5346808689088317No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2530.5304872934664094No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT2410.5053258408118763No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT2270.47597081271492075No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2010.421454331963432No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1920.4025832424725321No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1760.3690346389331544No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1710.3585507003270989No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1650.34596997399983226No Hit
GTGTCAGCCGCCGCGGTAATACATAGGGGGCAAGCGTTATCCGGAATTAT1540.3229053090665101No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT1520.3187117336240879No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT1460.3061310072968213No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1430.29984064413318795No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1360.2851631300847102No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1350.2830663423634991No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT1350.2830663423634991No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1280.2683888283150214No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1240.26000167743017694No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC1200.25161452654533256No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT1160.2432273756604881No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1160.2432273756604881No Hit
GTGTCAGCAGCCGCGGTAATACATAGGGGGCAAGCGTTATCCGGAATTAT1140.23903380021806594No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1100.2306466493332215No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1100.2306466493332215No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1090.2285498616120104No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1090.2285498616120104No Hit
GTGCCAGCAGCCGCGGTAATACATAGGGGGCAAGCGTTATCCGGAATTAT980.20548519667868825No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC970.20338840895747715No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC960.20129162123626604No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT950.19919483351505496No Hit
GTGCCAGCCGCCGCGGTAATACATAGGGGGCAAGCGTTATCCGGAATTAT930.19500125807263274No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT920.19290447035142164No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC920.19290447035142164No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT920.19290447035142164No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT920.19290447035142164No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC880.1845173194665772No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC870.1824205317453661No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT870.1824205317453661No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT840.17613016858173278No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT830.17403338086052167No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC820.17193659313931056No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT820.17193659313931056No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC810.16983980541809948No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC800.16774301769688837No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT780.16354944225446616No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC780.16354944225446616No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT770.16145265453325505No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT760.15935586681204394No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT720.15096871592719954No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT710.14887192820598844No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT680.1425815650423551No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT670.140484777321144No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC670.140484777321144No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC660.1383879895999329No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT660.1383879895999329No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT660.1383879895999329No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT650.13629120187872182No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT630.13209762643629958No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC630.13209762643629958No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC610.1279040509938774No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT610.1279040509938774No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT590.12371047555145517No Hit
GTGTCAGCAGCCGCGGTAAAACATAGGGGGCAAGCGTTGTCCGGAATCAC580.12161368783024405No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT540.11322653694539964No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC540.11322653694539964No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC530.11112974922418856No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT530.11112974922418856No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT520.10903296150297744No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT520.10903296150297744No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC520.10903296150297744No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT520.10903296150297744No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT520.10903296150297744No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC510.10693617378176633No Hit
GTGTCAGCCGCCGCGGTAAAACATAGGGGGCAAGCGTTGTCCGGAATCAC510.10693617378176633No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACGAGCGTTGTCCGGAATTAC510.10693617378176633No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC500.10483938606055522No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC500.10483938606055522No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT500.10483938606055522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)