FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61456
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA25974.225787555324134No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA23413.8092293673522524No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15022.4440249934912783No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14782.4049726633689144No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14072.2894428534235876No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14052.2861884925800573No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13722.2324915386618067No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12792.0811637594376466No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11811.9217000781046605No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11741.910309815152304No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11701.9038010934652432No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11581.8842749284040612No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11551.8793933871387658No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11141.8126789898463942No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10811.7589820359281436No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10641.7313199687581358No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10171.654842488935173No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10031.632061963030461No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9761.5881280916428016No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9561.555584483207498No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9431.5344311377245508No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8751.4237828690445198No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8411.368458734704504No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7461.2138765946368133No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7441.210622233793283No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6621.0771934392085394No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4500.7322311897943243No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4290.698060400937256No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4200.6834157771413695No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4180.6801614162978391No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4000.6508721687060661No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3800.6183285602707628No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3410.5548685238219214No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3360.5467326217130956No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3320.5402239000260348No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3240.5272064566519136No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3170.5158161936995573No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3150.5125618328560271No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3000.4881541265295496No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2950.4800182244207238No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2880.4686279614683676No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2840.4621192397813069No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2800.4556105180942463No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2740.4458474355636553No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2710.44096589429835975No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2650.43120281176776876No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2570.4181853683936475No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2530.4116766467065869No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2450.3986592033324655No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2450.3986592033324655No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2390.3888961208018745No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2320.37750585784951834No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2190.3563525123665712No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2080.3384535277271544No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1790.2912652954959646No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1610.2619760479041916No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1570.255467326217131No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1540.2505857849518355No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1540.2505857849518355No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1520.24733142410830514No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1500.2440770632647748No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1480.24082270242124448No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1440.2343139807341838No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1440.2343139807341838No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1380.2245508982035928No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1380.2245508982035928No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1370.22292371778182765No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1360.22129653736006247No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1350.2196693569382973No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1350.2196693569382973No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1330.21641499609476697No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1330.21641499609476697No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1330.21641499609476697No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1300.21153345482947147No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.206651913564176No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.206651913564176No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1260.20502473314241085No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1260.20502473314241085No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1250.2033975527206457No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1220.1985160114553502No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1210.196888831033585No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1200.19526165061181985No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.19363447019005467No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1170.19038010934652436No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1160.18875292892475917No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1150.18712574850299402No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.18549856808122883No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1140.18549856808122883No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1130.18387138765946368No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1130.18387138765946368No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1120.18224420723769852No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1100.17898984639416818No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.17898984639416818No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1100.17898984639416818No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.17898984639416818No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1080.17573548555063784No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1070.1741083051288727No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1040.1692267638635772No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1030.16759958344181203No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1030.16759958344181203No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1030.16759958344181203No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1010.1643452225982817No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA990.16109086175475137No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA980.1594636813329862No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA960.15620932048945588No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA960.15620932048945588No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA960.15620932048945588No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA940.15295495964592554No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA940.15295495964592554No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA940.15295495964592554No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA910.14807341838063004No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA910.14807341838063004No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.14481905753709973No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.14481905753709973No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.14481905753709973No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA890.14481905753709973No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA880.14319187711533454No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.1415646966935694No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA870.1415646966935694No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA870.1415646966935694No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA860.13993751627180423No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.13831033585003905No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA840.1366831554282739No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA700.11390262952356157No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA660.10739390783650091No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA650.10576672741473574No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA630.10251236657120542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)