FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63220
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA27504.3498892755457135No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA25324.005061689338817No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA20353.218918063903828No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA18412.9120531477380576No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14572.304650427080038No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13802.182853527364758No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA10761.701993040177159No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA10761.701993040177159No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA10751.7004112622587788No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9961.5754508067067383No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9241.4615627965833595No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9231.4599810186649793No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9091.4378361278076557No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8981.420436570705473No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8701.3761467889908259No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA8531.3492565643783614No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7931.2543498892755456No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7701.2179689971527998No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7571.1974058842138562No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7561.195824106295476No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7511.187915216703575No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7061.1167352103764632No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA6981.104080987029421No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA6691.0582094273963936No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA6671.0550458715596331No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA6310.9981018664979437No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5850.9253400822524518No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5820.9205947484973109No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5810.9190129705789307No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5770.9126858589054097No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5670.8968680797216071No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5630.890540968048086No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5440.8604871875988611No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5420.8573236317621006No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5130.811452072129073No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5070.8019614046187916No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4980.7877254033533692No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4890.7734894020879468No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4890.7734894020879468No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4810.7608351787409048No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4780.7560898449857639No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4620.7307813982916798No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4580.7244542866181588No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4500.7118000632711168No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4480.7086365074343562No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4470.7070547295159759No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4360.6896551724137931No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4330.6849098386586523No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4180.6611831698829485No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4140.6548560582094274No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4020.6358747231888643No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3970.627965833596963No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3800.6010756089844985No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3790.5994938310661183No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3700.585257829800696No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3680.5820942739639355No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3620.5726036064536538No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3590.5678582726985132No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3450.5457133818411894No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3370.5330591584941474No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3280.5188231572287251No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3270.5172413793103449No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3180.5030053780449225No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2980.4713698196773173No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2970.46978804175893707No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2940.46504270800379627No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2870.45397026257513445No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2850.4508067067383739No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.4334071496361911No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2580.40809870294210693No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2570.4065169250237267No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2420.3827902562480228No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.37804492249288196No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2330.36855425498260047No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2240.3543182537171781No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2190.3464093641252768No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2170.3432458082885163No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2050.3242644732679532No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1960.31002847200253086No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1630.2578298006959823No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1590.25150268902246126No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1570.24833913318570072No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1510.23884846567541915No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1490.23568490983865864No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1470.23252135400189813No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1460.2309395760835179No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1310.20721290730781397No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1240.19614046187915218No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1210.1913951281240114No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1210.1913951281240114No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1170.18506801645049037No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.18032268269534957No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.17399557102182853No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1090.1724137931034483No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1070.16925023726668775No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1060.1676684593483075No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1030.1629231255931667No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA950.15026890224612463No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA950.15026890224612463No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA910.1439417905726036No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.14077823473584308No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA870.13761467889908258No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.13761467889908258No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA870.13761467889908258No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.1360329009807023No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.13445112306232204No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA840.1328693451439418No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA820.12970578930718127No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA810.128124011388801No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.1249604555520405No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA790.1249604555520405No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA780.12337867763366024No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.12179689971527999No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.12021512179689971No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA750.11863334387851945No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA710.11230623220499841No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA710.11230623220499841No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA700.11072445428661816No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.10756089844985764No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA670.10597912053147739No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA670.10597912053147739No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA660.10439734261309712No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA650.10281556469471685No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA650.10281556469471685No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA640.1012337867763366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)