FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61887
Sequences flagged as poor quality0
Sequence length239
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT23873.85702974776609No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT21303.4417567502060207No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT21183.4223665713316205No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT21023.396512999499087No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT18903.053953172718018No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT17322.79864915087175No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT17192.7776431237578167No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT17122.766332186081083No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT16972.742094462488083No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT16032.590204727971949No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT15772.548192673744082No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT14642.3656018226768145No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC10481.6934089550309437No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC9951.6077689983356764No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC9821.5867629712217428No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC9331.507586407484609No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC8921.441336629663742No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC8861.4316415402265419No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8851.4300256919870085No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC8571.384781941280075No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC8501.3734710036033417No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8321.3443857352917414No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8301.3411540388126748No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8011.2942944398662077No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC7261.173105821901207No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC7011.1327096159128736No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC6491.0486855074571395No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC6381.0309111768222727No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC6361.027679480343206No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC5640.9113384070968055No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC5590.9032591658991387No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC5350.8644788081503384No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC4270.6899671982807375No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4230.683503805322604No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4050.6544185370110039No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3930.6350283581366037No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3850.6221015722203371No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3780.6107906345436036No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3730.6027113933459369No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3380.54615670496227No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC3290.5316140708064698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC3280.5299982225669365No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC3230.5219189813692698No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3020.48798616833906955No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC2950.4766752306623362No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT2940.4750593824228029No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC2890.4669801412251361No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT2850.46051674826700273No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT2750.44435826587166927No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT2750.44435826587166927No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2600.42012054227866913No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2520.40719375636240246No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC2480.4007303634042691No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC2470.3991145151647358No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2370.3829560327694023No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2320.3748767915717356No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC2320.3748767915717356No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC2300.3716450950926689No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC2270.3667975503740689No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC2260.3651817021345355No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2240.3619500056554688No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC2110.34094397854153535No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2090.33771228206246867No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC2000.32316964790666863No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1990.32155379966713526No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC1870.3021636207927351No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC1860.3005477725532018No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC1600.2585357183253349No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1600.2585357183253349No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1340.21652366409746795No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1300.21006027113933456No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1230.1987493334626012No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1220.19713348522306784No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1190.19228594050446782No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1180.19067009226493448No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1160.1874383957858678No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1130.18259085106726775No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1120.1809750028277344No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC1110.1793591545882011No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1100.17774330634866775No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1090.17612745810913438No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1090.17612745810913438No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1080.17451160986960104No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1070.1728957616300677No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1070.1728957616300677No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1060.17127991339053436No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTATCCGGATTTAT1060.17127991339053436No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC1040.16804821691146768No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1000.16158482395333432No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC980.1583531274742676No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC980.1583531274742676No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC960.15512143099520093No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC960.15512143099520093No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC920.14865803803706756No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC920.14865803803706756No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC910.1470421897975342No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT900.14542634155800088No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT890.14381049331846754No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC880.1421946450789342No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC850.13734710036033415No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC840.1357312521208008No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC820.13249955564173413No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC810.1308837074022008No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC810.1308837074022008No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC790.1276520109231341No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT750.12118861796500072No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC740.11957276972546739No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC710.11472522500686735No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC700.11310937676733401No Hit
GTGTCAGCCGCCGCGGTAACACCGGCAGCTCTAGTGGTAGCAGTTTTTAT680.10987768028826732No Hit
GTGTCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT680.10987768028826732No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT660.10664598380920065No Hit
GTGCCAGCCGCCGCGGTAACACCGGCAGCTCTAGTGGTAGCAGTTTTTAT650.10503013556966728No Hit
GTGTCAGCAGCCGCGGTAACACCGGCAGCTCTAGTGGTAGCAGTTTTTAT620.10018259085106727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)