FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003937833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003937833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38916
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13623.499845821769966No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA11773.0244629458320484No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11632.988488025490801No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10942.8111830609517936No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6411.6471374241957035No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6261.6085928666872238No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6051.554630486175352No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5531.4210093534792887No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5521.4184397163120568No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5441.3978826189742009No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5391.385034433138041No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5221.341350601295097No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5211.3387809641278652No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5121.3156542296227773No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4891.2565525747764417No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4851.2462740261075136No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4701.2077294685990339No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4571.174324185425018No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4511.158906362421626No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4511.158906362421626No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4301.1049439819097542No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4291.1023743447425225No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4181.0741083359029706No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3941.0124370438894028No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3911.0047281323877069No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3420.8788159111933395No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3260.8377017165176278No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3090.794017884674684No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3070.78887861034022No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3060.786308973172988No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3030.778600061671292No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2990.7683215130023641No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2960.7606126015006681No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2930.7529036899989722No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2900.7451947784972762No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2800.7194984068249562No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2600.6681056634803166No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2600.6681056634803166No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2480.6372700174735327No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2450.6295611059718368No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2440.6269914688046048No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2430.6244218316373727No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2340.6012950971322849No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2330.598725459965053No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2220.5704594511255011No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2170.5576112652893411No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2160.5550416281221091No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.5499023537876452No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2070.5319148936170213No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1990.5113577962791654No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1940.49850961044300546No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1840.4728132387706856No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1820.46767396443622167No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.46510432726898965No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1800.46253469010175763No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1780.45739541576729364No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1750.4496865042655977No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1740.4471168670983657No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1710.43940795559666973No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1670.4291294069277418No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1580.4060026724226539No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1530.393154486586494No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1430.3674581149141741No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1400.3597492034124781No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1290.3314831945729263No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA1230.3160653715695344No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA1230.3160653715695344No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.3134957344023024No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1040.26724226539212664No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA980.25182444238873475No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.2466851680542707No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.2389762565525748No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA920.2364066193853428No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.23126734505087881No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA900.23126734505087881No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA890.22869770788364682No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA860.22098879638195087No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA840.21584952204748692No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA830.2132798848802549No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA830.2132798848802549No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA810.20814061054579094No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA790.20300133621132696No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA790.20300133621132696No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.20043169904409497No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA780.20043169904409497No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.197862061876863No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.19529242470963099No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA760.19529242470963099No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA760.19529242470963099No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA750.19272278754239902No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA750.19272278754239902No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA740.19015315037516703No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA710.18244423887347105No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA710.18244423887347105No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA710.18244423887347105No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA710.18244423887347105No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.1773049645390071No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.1773049645390071No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA690.1773049645390071No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA690.1773049645390071No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA670.17216569020454311No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA660.16959605303731112No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA660.16959605303731112No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA650.16702641587007916No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA630.16188714153561518No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA630.16188714153561518No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA630.16188714153561518No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA630.16188714153561518No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA620.15931750436838318No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA620.15931750436838318No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA610.1567478672011512No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA610.1567478672011512No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA610.1567478672011512No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA600.1541782300339192No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA590.15160859286668724No Hit
GGACTACGCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA570.14646931853222325No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA540.13876040703052728No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA530.1361907698632953No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA530.1361907698632953No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA530.1361907698632953No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA520.13362113269606332No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA500.12848185836159934No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA490.12591222119436737No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCATCTCA490.12591222119436737No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA470.12077294685990338No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA460.1182033096926714No Hit
GGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA460.1182033096926714No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA450.11563367252543941No Hit
GGACTACGAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA450.11563367252543941No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA440.11306403535820743No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA430.11049439819097544No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA430.11049439819097544No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA420.10792476102374346No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCTTCA420.10792476102374346No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA420.10792476102374346No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA410.10535512385651145No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA410.10535512385651145No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA410.10535512385651145No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA410.10535512385651145No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA410.10535512385651145No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA410.10535512385651145No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCATCTCA400.10278548668927946No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA390.10021584952204748No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA390.10021584952204748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)