FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003938087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003938087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58005
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA27924.813378157055427No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA26824.623739332816137No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA17663.044565123696233No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA16772.891130074993535No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11381.9618998362210154No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11271.9429359537970865No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA11071.908456167571761No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10971.8912162744590981No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10301.7757089906042582No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10211.760193086802862No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10041.7308852685113352No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9931.7119213860874065No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9691.6705456426170158No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8991.549866390828377No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7761.3378157055426256No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7551.3016119300060338No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7421.2792000689595724No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6831.1774846995948627No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6821.175760710283596No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6581.1343849668132058No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6561.1309369881906732No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6531.1257650202568743No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6381.0999051805878803No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6151.0602534264287562No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6121.0550814584949573No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6081.0481855012498922No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5911.0188776829583657No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5730.9878458753555728No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5620.9688819929316438No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5440.937850185328851No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5220.8999224204809929No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5210.8981984311697268No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5090.8775105594345314No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4760.8206189121627445No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4710.8119989656064133No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4700.810274976295147No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4640.7999310404275494No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4610.7947590724937506No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4100.7068356176191708No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4010.6913197138177742No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3890.6706318420825791No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3830.6602879062149815No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3790.6533919489699164No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3570.6154641841220584No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3470.5982242910093958No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3250.5602965261615378No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3230.5568485475390053No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3220.555124558227739No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3190.5499525902939402No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3010.5189207826911473No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2900.49995690026721834No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2720.4689250926644255No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2590.44651323161796397No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2530.43616929575036634No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2320.3999655202137747No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2280.39306956296870954No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2090.3603137660546505No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2050.3534178088095854No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2010.3465218515645203No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1980.3413498836307215No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1960.33790190500818895No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1810.31204206533919493No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1700.29307818291526594No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1580.2723903111800707No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1510.26032238600120683No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA1500.2585983966899405No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1430.24653047151107663No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1380.2379105249547453No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1370.236186535643479No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1330.22929057839841394No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1280.22067063184208258No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1270.2189466425308163No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1240.2137746745970175No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1230.21205068528575124No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1220.21032669597448495No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1220.21032669597448495No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1200.2068787173519524No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.20515472804068613No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1140.19653478148435477No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1110.19136281355055598No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1110.19136281355055598No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1110.19136281355055598No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1050.18101887768295835No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1050.18101887768295835No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1000.17239893112662702No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA950.16377898457029566No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA950.16377898457029566No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA940.1620549952590294No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.16033100594776312No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA920.15860701663649684No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA920.15860701663649684No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA900.1551590380139643No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA890.15343504870269806No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA890.15343504870269806No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA890.15343504870269806No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA870.14998707008016549No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA860.1482630807688992No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA850.14653909145763297No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA810.13964313421256788No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA800.1379191449013016No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA800.1379191449013016No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA790.13619515559003534No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA770.1327471769675028No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA770.1327471769675028No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.13102318765623652No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA750.12929919834497025No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA740.127575209033704No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA730.12585121972243773No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA730.12585121972243773No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCA720.12412723041117144No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA720.12412723041117144No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA700.1206792517886389No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.11895526247737265No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.11895526247737265No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA680.11723127316610638No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA670.11550728385484009No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA670.11550728385484009No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA640.11033531592104129No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA620.10688733729850874No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA620.10688733729850874No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA610.10516334798724247No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA610.10516334798724247No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA610.10516334798724247No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA600.1034393586759762No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA590.10171536936470994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)