FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003938108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003938108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63235
Sequences flagged as poor quality0
Sequence length239
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT697911.036609472602198No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT61499.72404522811734No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT60489.564323554993278No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT60229.523207084684115No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC29014.587649244880209No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC26994.268205898632087No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC25794.078437574128252No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC25123.972483592946944No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC15632.4717324266624496No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC15052.3800110698189294No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC14852.348383015734957No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC13672.161777496639519No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT5850.9251205819561952No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT5650.8934925278722228No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT5450.8618644737882502No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5080.803352573732901No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT4820.7622361034237368No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4760.7527476871985451No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4680.7400964655649561No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4520.7147940222977781No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC2970.4696766031469914No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT2770.43804854906301893No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT2590.40958330038744367No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT2550.4032576895706492No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT2390.3779552463034712No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC2390.3779552463034712No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC2300.36372262196568356No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCGAGCGTTGTTCGGAATTAC2230.3526528030362932No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1810.286233889459951No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1800.2846524867557524No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1790.28307108405155373No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1670.2640942516011702No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1560.24669882185498537No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1360.2150707677710129No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1330.210326559658417No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1250.19767533802482803No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1240.1960939353206294No Hit
GTGCCAGCAGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1240.1960939353206294No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1220.19293112991223216No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC1210.1913497272080335No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1200.1897683245038349No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1190.18818692179963628No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1160.1834427136870404No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1140.18027990827864315No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT1080.1707914920534514No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCAT1080.1707914920534514No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1040.16446588123665692No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC1040.16446588123665692No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT1030.1628844785324583No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCAT1030.1628844785324583No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1010.15972167312406105No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCAT1010.15972167312406105No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1000.15814027041986242No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC1000.15814027041986242No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC990.1565588677156638No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATCAT970.15339606230726655No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT970.15339606230726655No Hit
GTGTCAGCAGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC960.15181465960306792No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGTTCGGAATTAC940.14865185419467067No Hit
GTGCCAGCCGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC900.1423262433778762No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT870.13758203526528032No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATGAT830.13125642444848581No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT800.12651221633588994No Hit
GTGTCAGCCGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC800.12651221633588994No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT790.1249308136316913No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT770.12176800822329406No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC770.12176800822329406No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT690.10911678658970508No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC690.10911678658970508No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC680.10753538388550644No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC660.10437257847710919No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTAC660.10437257847710919No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTAC650.10279117577291057No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTAC640.10120977306871196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)