FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003938498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003938498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106848
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT64556.041292303084756No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT48734.560684336627733No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC45294.238731656184487No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT45094.220013477088949No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC43064.030023959269243No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT41103.8465858041329737No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT34713.248539982030548No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC32903.0791404612159328No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC32243.0173704702006585No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC31892.984613656783468No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC29452.7562518718179096No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC29082.721623240491165No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC27422.5662623539982032No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT27042.5306978137166816No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT24232.2677073974243784No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT23772.224655585504642No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC22392.095500149745433No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGCGGCAAGCGTTGTCCGGAATTAT21602.0215633423180592No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT20121.883048817011081No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT18521.7333033842467804No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT17701.6565588499550765No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC16241.519916142557652No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC15691.4684411500449235No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC14701.3757861635220126No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT10040.9396525905959869No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8550.8002021563342319No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT8490.7945867026055705No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT7620.713162623539982No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT7330.6860212638514526No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT6720.628930817610063No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT6420.6008535489667565No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC6330.5924303683737646No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT6310.5905585504642108No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT5930.5549940101826893No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC5710.5344040131775981No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC5430.5081985624438454No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT5280.4941599281221923No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT5270.49322401916741543No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC4960.4642108415693322No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC4210.3940176699610662No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT3880.36313267445342917No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC3710.3472222222222222No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT3660.34254267744833783No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT3590.3359913147648997No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC3580.3350554058101228No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT3530.3303758610362384No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT3470.3247604073075771No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3260.3051063192572626No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3150.294811320754717No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3130.29293950284516324No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT3120.2920035938903864No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC3020.2826445043426175No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT2150.20122042527702905No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1990.18624588200059897No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1860.17407906558849956No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1750.16378406708595386No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1720.16097634022162324No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1610.15068134171907757No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1610.15068134171907757No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTATCCGGATTTAT1490.13945043426175502No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1460.13664270739742437No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1450.1357067984426475No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1340.12541179994010182No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1310.12260407307577117No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1310.12260407307577117No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1300.12166816412099431No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1290.12073225516621743No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1290.12073225516621743No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCGAGCGTTATCCGGATTCAT1270.11886043725666366No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1270.11886043725666366No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1250.11698861934710991No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1250.11698861934710991No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1210.1132449835280024No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1190.11137316561844862No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1130.10575771188978736No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC1090.10201407607067985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)