FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003938967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003938967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23958
Sequences flagged as poor quality0
Sequence length239
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6562.738125052174639No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5642.35411970949161No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3311.381584439435679No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3121.3022789882294015No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3021.2605392770682027No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2991.248017363719843No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2891.2062776525586443No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2891.2062776525586443No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2821.177059854745805No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2761.1520160280490859No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2421.0101010101010102No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2401.0017530678687703No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2330.9725352700559311No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2200.9182736455463728No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.8765339343851741No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA1930.8055764254111362No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA1920.8014024542950162No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1870.7805325987144168No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1810.7554887720176977No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.7220970030887387No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA1730.7220970030887387No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.7012271475081392No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1650.6887052341597797No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.6678353785791802No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.6678353785791802No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.6511394941147007No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.6469655229985809No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.6302696385341013No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.6219216963018616No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.6177477251857417No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.6052258118373821No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1390.5801819851406628No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1380.5760080140245429No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.5426162450955839No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1280.5342683028633441No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.49670256281826536No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1190.49670256281826536No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.4841806494699057No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.4841806494699057No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1150.48000667835378585No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1120.46748476500542613No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1120.46748476500542613No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1070.4466149094248268No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1020.4257450538442274No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1000.4173971116119876No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA980.40904916937974783No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA950.3965272560313883No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA950.3965272560313883No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.3881793137991485No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.3881793137991485No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA920.38400534268302866No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA920.38400534268302866No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA890.371483429334669No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA890.371483429334669No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.3547875448701895No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA850.3547875448701895No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.3547875448701895No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA840.3506135737540696No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA820.34226563152182987No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA810.33809166040571No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA790.3297437181734702No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA760.3172218048251106No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA760.3172218048251106No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA760.3172218048251106No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA750.31304783370899075No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA740.30887386259287086No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA720.30052592036063114No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.29217797812839136No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA700.29217797812839136No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA690.28800400701227147No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA690.28800400701227147No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA690.28800400701227147No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA680.28383003589615163No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA660.27548209366391185No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA650.27130812254779196No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA630.26296018031555224No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA620.25878620919943235No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA620.25878620919943235No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA610.25461223808331246No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA610.25461223808331246No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA600.25043826696719257No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA600.25043826696719257No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA580.24209032473495284No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA580.24209032473495284No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA580.24209032473495284No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA570.23791635361883295No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA560.23374238250271306No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA560.23374238250271306No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.23374238250271306No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA540.2253944402704733No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA540.2253944402704733No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA540.2253944402704733No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA530.22122046915435345No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA530.22122046915435345No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA530.22122046915435345No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA520.21704649803823356No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA520.21704649803823356No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA510.2128725269221137No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA510.2128725269221137No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA500.2086985558059938No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA500.2086985558059938No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA490.20452458468987392No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA480.20035061357375405No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA480.20035061357375405No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA470.19617664245763416No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA460.19200267134151433No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA460.19200267134151433No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA450.18782870022539444No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA450.18782870022539444No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA450.18782870022539444No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA440.18365472910927455No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA440.18365472910927455No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA430.1794807579931547No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA420.1753067868770348No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA420.1753067868770348No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA420.1753067868770348No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA420.1753067868770348No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA420.1753067868770348No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA410.17113281576091494No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA410.17113281576091494No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA400.16695884464479505No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA400.16695884464479505No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA400.16695884464479505No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA390.16278487352867518No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA370.15443693129643543No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA360.15026296018031557No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA360.15026296018031557No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA350.14608898906419568No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA340.14191501794807582No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA340.14191501794807582No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA310.12939310459971617No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA300.12521913348359628No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA290.12104516236747642No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA290.12104516236747642No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA280.11687119125135653No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA280.11687119125135653No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA280.11687119125135653No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA270.11269722013523666No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA270.11269722013523666No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA260.10852324901911678No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA260.10852324901911678No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA260.10852324901911678No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA250.1043492779029969No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA250.1043492779029969No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA240.10017530678687703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)