FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42918
Sequences flagged as poor quality0
Sequence length239
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA25685.983503425136306No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA22685.284496015657766No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12192.840300107181136No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10802.5164266741227457No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10282.395265389813132No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9862.297404352486136No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9772.27643413020178No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9202.1436227224008575No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8992.0946922037373596No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8732.034111561582553No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8161.90130015378163No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7701.794119017661587No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5731.3351041521040123No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5421.2628733864578965No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5401.2582133370613728No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5231.2186029171909223No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5131.195302670208304No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5001.1650123491309008No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4971.1580222750361155No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4781.1137518057691411No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4631.0788014352952142No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4541.057831213010858No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4501.0485111142178107No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4260.9925905214595274No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4210.9809403979682184No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA4120.9599701756838621No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3900.9087096323221026No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA3450.8038585209003215No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3150.7339577799524675No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2770.6454168414185191No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2620.610466470944592No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.5382357052984762No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.505615359522811No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2110.4916352113332401No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1890.4403746679714805No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1890.4403746679714805No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.4287245444801715No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.4287245444801715No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1780.41474439629060067No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1770.41241437159233885No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1710.39843422340276813No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1690.3937741740062444No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1690.3937741740062444No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.39144414930798266No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1670.38911412460972084No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1630.37979402581667365No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1600.37280395172188824No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.36814390232536465No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.36814390232536465No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1490.3471736800410084No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1450.3378535812479612No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1390.3238734330583904No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1380.3215434083601286No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1320.3075632601705578No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.2959131366792488No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1260.29358311198098697No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1250.2912530872827252No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.2889230625844634No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1200.2796029637914162No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1200.2796029637914162No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1200.2796029637914162No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA1180.2749429143948926No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA1160.27028286499836895No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1130.2632927909035836No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1100.2563027168087982No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.2563027168087982No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1080.2516426674122746No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1070.24931264271401277No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1060.24698261801575097No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1050.24465259331748918No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA1050.24465259331748918No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1050.24465259331748918No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1010.23533249452444197No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA980.22834242042965655No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA980.22834242042965655No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA960.22368237103313296No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.22368237103313296No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA950.22135234633487116No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.22135234633487116No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA940.21902232163660934No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA930.21669229693834755No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA930.21669229693834755No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA920.21436227224008575No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA910.21203224754182395No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.20970222284356216No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.20970222284356216No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.20970222284356216No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA900.20970222284356216No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA850.19805209935225315No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA830.19339204995572953No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA830.19339204995572953No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.19339204995572953No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA830.19339204995572953No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA810.18873200055920591No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.18640197586094412No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.18640197586094412No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.18640197586094412No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA790.18407195116268232No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA790.18407195116268232No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA780.18174192646442053No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA770.17941190176615873No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.1747518523696351No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA750.1747518523696351No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA710.1654317535765879No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA710.1654317535765879No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA660.1537816300852789No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA660.1537816300852789No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA650.1514516053870171No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA620.1444615312922317No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA620.1444615312922317No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA610.1421315065939699No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA610.1421315065939699No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA600.1398014818957081No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA600.1398014818957081No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA590.1374714571974463No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA590.1374714571974463No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA570.13281140780092268No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.1304813831026609No Hit
GGACTACGCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA560.1304813831026609No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA560.1304813831026609No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA540.1258213337061373No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA540.1258213337061373No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA530.12349130900787549No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.12349130900787549No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA530.12349130900787549No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA530.12349130900787549No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA520.12116128430961369No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA520.12116128430961369No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA510.11883125961135188No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA510.11883125961135188No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA510.11883125961135188No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA510.11883125961135188No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA500.11650123491309008No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA500.11650123491309008No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA490.11417121021482828No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA480.11184118551656648No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA480.11184118551656648No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA470.10951116081830467No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA470.10951116081830467No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA460.10718113612004287No Hit
GGACTACGCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA460.10718113612004287No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA450.10485111142178108No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA440.10252108672351927No Hit
GGACTACGAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA440.10252108672351927No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA430.10019106202525747No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCCTCA430.10019106202525747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)