FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89510
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA47765.335716679700592No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA41404.625181543961569No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA22672.532677913082337No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA19012.1237850519495027No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA18402.055636241760697No Hit
GGACTACGGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA17841.993073399620154No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA17381.9416824935761368No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17001.8992291364093397No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15391.7193609652552788No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14831.6567981231147357No Hit
GGACTACGGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA14081.5730086023907943No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13551.513797341079209No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13491.5070941794212938No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA13131.4668752094738018No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12791.428890626745615No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12571.404312367333259No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12291.3730309462629873No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12221.3652105909954195No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8310.9283878896212714No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7960.889286113283432No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7320.8177857222656687No Hit
GGACTACGCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA6920.7730979778795665No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6860.7663948162216512No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6850.7652776226119986No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6770.7563400737347782No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6610.7384649759803374No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6610.7384649759803374No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6140.6859568763266674No Hit
GGACTACGCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA6110.6826052954977098No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5910.6602614233046586No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5880.6569098424757011No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5860.6546754552563959No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5810.6490894872081331No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5720.6390347447212602No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5690.6356831638923025No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5580.6233940341861245No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5520.6166908725282092No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5480.6122220980895989No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5450.6088705172606412No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5390.6021673556027259No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5340.5965813875544632No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA5200.5809406770193274No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA5150.5753547089710648No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5150.5753547089710648No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5110.5708859345324545No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5020.5608311920455815No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4950.5530108367780135No Hit
GGACTACGAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4910.5485420623394034No Hit
GGACTACCGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4900.5474248687297509No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4870.5440732879007931No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4830.539604513462183No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4800.5362529326332254No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4780.5340185454139202No Hit
GGACTACGAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4680.5228466093173947No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4630.517260641269132No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4540.507205898782259No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4460.4982683499050385No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4400.49156518824712325No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4370.4882136074181656No Hit
GGACTACTGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4290.4792760585409451No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4250.4748072841023349No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4170.4658697352251145No Hit
GGACTACAGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA4110.4591665735671992No Hit
GGACTACTGGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA3870.432353926935538No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3600.402189699474919No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3000.3351580828957658No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2790.31169701709306225No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2650.2960563065579265No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2640.2949391129482739No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2640.2949391129482739No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2570.28711875768070605No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2510.28041559602279076No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2510.28041559602279076No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.28041559602279076No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2480.27706401519383306No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2280.25472014300078205No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2280.25472014300078205No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2250.25136856217182435No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA2240.25025136856217184No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2200.24578259412356163No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.24243101329460395No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2160.24131381968495141No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2140.2390794324656463No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2120.2368450452463412No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2070.23125907719807842No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2050.22902468997877332No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA2030.2267903027594682No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2030.2267903027594682No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1980.22120433471120549No Hit
GGACTACTCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1970.2200871411015529No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1950.21785275388224778No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.21785275388224778No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1940.21673556027259522No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1940.21673556027259522No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1890.2111495922243325No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1890.2111495922243325No Hit
GGACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1860.2077980113953748No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1860.2077980113953748No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1830.20444643056641718No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1810.20221204334711207No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1790.19997765612780696No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCA1760.1966260752988493No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.19327449446989162No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1710.1910401072505865No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1700.18992291364093397No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1690.1888057200312814No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1680.18768852642162887No Hit
GGACTACTAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1670.1865713328119763No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.1865713328119763No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1630.1821025583733661No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1620.18098536476371355No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1600.17875097754440844No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1600.17875097754440844No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1550.1731650094961457No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA1540.17204781588649312No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1530.17093062227684058No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCACCTCA1520.16981342866718802No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1500.1675790414478829No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA1490.16646184783823037No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1490.16646184783823037No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1480.1653446542285778No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1450.16199307339962016No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1420.1586414925706625No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1400.1564071053513574No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1380.1541727181320523No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1350.15082113730309463No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCA1340.14970394369344206No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1330.14858675008378952No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1320.14746955647413695No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1310.14635236286448441No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1250.1396492012065691No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCA1240.13853200759691653No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1200.13406323315830634No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA1200.13406323315830634No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA1140.12736007150039103No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1130.12624287789073846No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCA1020.11395374818456039No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA900.10054742486872975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)