FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003939410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76381
Sequences flagged as poor quality0
Sequence length239
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC60857.966640918553043No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC48006.28428535892434No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC47506.218824053102211No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC39455.1648970293659415No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC35734.677864914049305No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC27503.6003718202170694No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC26183.4275539728466504No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT24073.1513072622772675No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC21342.793888532488446No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT18562.4299236721174116No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT18472.4181406370694285No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC17712.318639452219793No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT15552.0358466110681976No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC14321.8748117987457613No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT14121.84862727641691No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC14041.8381534674853695No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC12461.6312957410874431No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT11081.4506225370183685No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC11001.440148728086828No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT10881.424438014689517No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC9401.2306725494560165No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT9381.2280540972231313No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC9331.2215079666409185No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8071.0565454759691546No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5250.6873437111323497No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4540.5943886568649271No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4060.5315458032756838No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3760.49226901978240667No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT3530.4621568191042275No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3470.45430146240557207No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC3340.4372815228918187No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3130.4097877744465246No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3000.39276783493277123No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC2730.35741872978882183No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC2630.3443264686243961No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2610.34170801639151094No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2530.33123420745997045No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2440.3194511724119873No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC2300.3011220067817913No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC2260.295885102316021No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2230.2919574239666933No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2170.28410206726803783No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1960.2566083188227439No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC1940.2539898665898587No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1740.22780534426100732No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1600.20947617863081133No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1550.20293004804859846No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1540.20162082193215589No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1500.19638391746638562No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC1450.18983778688417277No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1280.16758094290464906No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC1260.16496249067176394No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1250.16365326455532136No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1210.15841636008955107No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1180.15448868174022334No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1180.15448868174022334No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC1090.1427056466922402No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1090.1427056466922402No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1020.13354106387714224No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC980.12830415941137194No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT890.1165211243633888No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC880.11521189824694622No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT880.11521189824694622No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGCTTTAT880.11521189824694622No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT850.11128421989761851No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC840.10997499378117594No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC810.10604731543184824No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT770.10081041096607794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)