FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003939535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98463
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA44694.538760752770076No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA40534.116267024161361No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA30393.086438560677615No Hit
GGACTACGGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA27692.8122238810517657No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA20632.095203274326397No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA18661.8951281191919807No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA18591.8880188497201993No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA17161.7427866305109534No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA16931.7194276022465291No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA15691.5934919716035465No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA14821.5051339081685506No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA14491.4716187806587244No Hit
GGACTACGCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA14001.4218538943562558No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA13971.4188070645826352No Hit
GGACTACGCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA13631.384276327148269No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA12911.3111524125813758No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA12681.2877933843169516No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12671.2867777743924114No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA12121.2309192285427013No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA12051.2238099590709202No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA11191.1364675055604643No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA11141.1313894559377635No Hit
GGACTACGAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA10351.051156271899089No Hit
GGACTACGAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9800.9952977260493789No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9390.9536577191432315No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9130.9272518611051868No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8900.9038928328407626No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8900.9038928328407626No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8890.9028772229162223No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8740.8876430740481197No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8690.8825650244254187No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8480.8612372160100749No Hit
GGACTACCCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA7480.7596762235560566No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7410.7525669540842753No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7160.7271767059707708No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6920.7028020677818063No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6870.6977240181591055No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6860.6967084082345653No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6810.6916303586118644No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6500.6601464509511187No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6490.6591308410265786No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6200.6296781532149133No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6180.6276469333658328No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6110.6205376638940515No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6040.6134283944222703No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6000.6093659547241096No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5970.606319124950489No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5920.6012410753277881No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5910.600225465403248No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5850.5941318058560069No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5460.5545230187989397No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5240.5321796004590558No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5160.5240547210627342No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5130.5210078912891136No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5050.5128830118927922No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4920.4996800828737698No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4860.4935864233265287No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4550.4621025156657831No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4510.4580400759676224No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4460.45296202634492144No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4440.4509308064958411No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4060.4123376293633141No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4010.4072595797406132No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3870.3930410407970507No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3710.37679128200440776No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3420.34733859419274243No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3390.3442917644191219No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3210.3260107857773986No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3210.3260107857773986No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3130.3178859063810771No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3110.3158546865319968No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3060.3107766369092959No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2980.3026517575129744No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2960.30062053766389407No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2830.28741760864487165No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2730.27726150939946986No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2720.27624589947492967No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2510.25491809105958585No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2360.2396839421914831No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2350.2386683322669429No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2270.23054345287062145No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA2220.22546540324792055No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2200.22343418339884016No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2180.22140296354975983No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2170.2203873536252196No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2110.21429369407797855No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2090.21226247422889816No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2090.21226247422889816No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2070.21023125437981782No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA2060.2092156444552776No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2030.20616881468165704No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA2020.20515320475711687No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2010.2041375948325767No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2000.20312198490803654No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.1980439352853356No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.1980439352853356No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.1980439352853356No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1950.1980439352853356No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1900.1929658856626347No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1880.19093466581355434No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1800.18280978641723286No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1790.1817941764926927No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1690.17163807724729085No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1580.16046636807734885No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1530.15538831845464796No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1530.15538831845464796No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1520.15437270853010776No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1470.14929465890740684No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1450.14726343905832648No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1450.14726343905832648No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1440.1462478291337863No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1420.14421660928470595No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1400.14218538943562556No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1390.1411697795110854No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1370.13913855966200503No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1340.13609172988838447No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1330.1350761199638443No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1320.1340605100393041No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1270.1289824604166032No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1250.12695124056752283No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1230.12492002071844245No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1210.12288880086936209No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1190.12085758102028173No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1180.11984197109574155No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1160.11781075124666117No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1130.11476392147304064No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1130.11476392147304064No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1110.11273270162396028No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1080.10968587185033972No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1070.10867026192579952No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1060.10765465200125936No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1030.1046078222276388No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1020.10359221230309863No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCGCTTTCGAGCTTCA1000.10156099245401827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)