FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53683
Sequences flagged as poor quality0
Sequence length239
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT586210.91965799228806No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT53679.997578376767319No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT50299.367956336270328No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT48589.049419741817708No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC19363.606355829592236No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC17943.3418400610994174No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC16263.0288918279529833No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC15582.90222230501276No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACGAGCGTTGTCCGGAATTAC13452.505448652273532No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACGAGCGTTGTCCGGAATTAC12122.2576979676992717No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACGAGCGTTGTCCGGAATTAC11212.08818434141162No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACGAGCGTTGTCCGGAATTAC11142.075144831697185No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT10721.9969077734105767No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT10221.9037684183074717No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT8911.6597433079373356No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT8631.607585269079597No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC7641.4231693459754484No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT7051.3132649069537843No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6691.2462045712795484No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6681.2443417841774864No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC6251.1642419387888159No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6231.1605163645846917No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5741.0692397965836484No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5731.0673770094815864No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4900.9127656800104316No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4610.8587448540506306No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4170.776782221559898No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC3980.7413892666207179No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2270.42285267216809785No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2260.42098988506603585No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2180.40608758824953894No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2150.40049922694335266No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC1890.3520667622897379No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1830.3408900396773653No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT1810.3371644654732411No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT1720.3203993815546821No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1650.3073598718402474No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC1570.2924575750237505No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC1440.26824134269694316No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC1400.26079019428869477No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1210.22539723934951475No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1150.21422051673714212No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1150.21422051673714212No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1140.21235772963508No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT730.13598345845053367No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC730.13598345845053367No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT570.10617886481754No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC540.1005905035113537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)