FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56637
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA15782.7861645214259227No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA14452.5513356992778573No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA13902.4542260359835444No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13272.3429913307555132No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10851.9157088122605364No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA9851.7391457880890586No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8061.4230979748221126No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA8011.4142698236135387No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7911.396613521196391No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7401.3065663788689372No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7391.3048007486272224No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7321.292441336935219No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA7261.2818475554849302No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7031.2412380599254904No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6601.1653159595317548No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6331.1176439430054557No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA6301.1123470522803114No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6061.0699719264791567No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5981.0558468845454385No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5831.0293624309197167No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5681.002877977293995No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5671.0011123470522802No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5600.9887529353602768No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5580.9852216748768473No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5540.9781591539099882No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5490.9693310027014143No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5330.9410809188339778No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5230.92342461641683No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5210.9198933559334004No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA5070.8951745325493934No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4620.8157211716722284No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4470.7892367180465067No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA4250.7503928527287815No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4210.7433303317619224No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4190.7397990712784929No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4180.7380334410367781No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4060.7168458781362007No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3880.6850645337853347No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3870.6832989035436199No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3840.6780020128184756No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3650.6444550382258947No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3620.6391581475007504No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3550.6267987358087469No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3540.6250331055670322No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3520.6215018450836026No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3510.6197362148418878No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3480.6144393241167435No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3430.6056111729081696No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3420.6038455426664548No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3400.6003142821830253No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3360.5932517612161661No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA3330.5879548704910218No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3300.5826579797658774No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3280.5791267192824479No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3220.5685329378321592No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA3040.5367515934812932No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA2980.5261578120310044No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA2960.5226265515475749No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2940.5190952910641453No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA2920.5155640305807158No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2880.5085015096138567No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA2790.49261083743842365No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCATATGA2770.4890795769549941No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2770.4890795769549941No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2640.466126383812702No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2640.466126383812702No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2410.425516888253262No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2360.41668873704468806No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2210.3902042834189664No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2160.38137613221039246No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.3778448717269629No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.3637198297932447No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1930.3407666366509526No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1640.2895633596412239No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1550.27367268746579093No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA1530.2701414269823614No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA1440.2542507548069283No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1430.25248512456521355No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA1400.2471882338400692No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA1150.20304747779719973No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1060.1871568056217667No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1020.18009428465490757No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA990.1747973939297632No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA980.17303176368804846No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA970.17126613344633368No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGA920.16243798223775976No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.1571410915126154No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGACTGA890.1571410915126154No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA870.15360983102918588No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA860.1518442007874711No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.1518442007874711No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA860.1518442007874711No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA850.15007857054575632No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA840.14831294030404152No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA830.14654731006232674No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA810.1430160495788972No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA800.1412504193371824No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGTCTCA780.13771915885375285No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA770.13595352861203808No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA760.1341878983703233No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA750.13242226812860852No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA740.13065663788689372No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA730.12889100764517894No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA720.12712537740346416No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA710.12535974716174939No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA700.1235941169200346No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA680.12006285643660504No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA680.12006285643660504No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA670.11829722619489028No Hit
GGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGAGCCTCA660.1165315959531755No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA650.1147659657114607No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA640.11300033546974592No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA640.11300033546974592No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA620.10946907498631638No Hit
GGACTACGGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA620.10946907498631638No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGAGCTTCA620.10946907498631638No Hit
GGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA610.1077034447446016No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA600.10593781450288679No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCATCTCA600.10593781450288679No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA600.10593781450288679No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA580.10240655401945725No Hit
GGACTACGGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA580.10240655401945725No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCA570.10064092377774247No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA570.10064092377774247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)