FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56637
Sequences flagged as poor quality0
Sequence length239
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT44887.924148524815933No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC40497.149036848703144No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT30815.439906774723237No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT30635.408125430372372No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC28355.005561735261402No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC27904.926108374384237No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT23424.1351060260960155No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC23024.064480816427424No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC22003.8843865317725164No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC15522.740258135141339No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC15502.736726874657909No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC11652.0569592315977188No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC8121.4336917562724014No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC6061.0699719264791567No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC5891.0399562123700055No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC5300.9357840281088334No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC4860.8580962974733831No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCGAGCGTTGTTCGGAATTAC4260.7521584829704964No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3600.6356268870173208No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC3580.6320956265338913No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC3380.5967830216995956No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCGAGCGTTGTTCGGAATTAC3200.5650016773487296No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCGAGCGTTGTTCGGAATTAC3090.545579744689867No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC3060.5402828539647228No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC2980.5261578120310044No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2780.4908452071967088No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2600.4590638628458428No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC2460.43434503946183595No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCGAGCGTTGTTCGGAATTAC2320.409626216077829No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT2220.3919699136606811No Hit
GTGCCAGCCGCCGCGGTAATACGTGGGTCGCAAGCGTTATCCGGAATCAT2190.38667302293553685No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2180.38490739269382207No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2020.3566573088263856No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1980.3495947878595264No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1980.3495947878595264No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1860.3284072249589491No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1790.31604781326694564No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1700.30015714109151265No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1650.2913289898829387No Hit
GTGTCAGCCGCCGCGGTAATACGTGGGTCGCAAGCGTTATCCGGAATCAT1610.2842664689160796No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1490.2630789060155022No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1460.2577820152903579No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1410.24895386408178402No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1370.24189134311492486No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1360.24012571287321008No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1320.233063191906351No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1290.2277663011812066No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1270.22423504069777705No Hit
GTGCCAGCAGCCGCGGTAATACGTGGGTCGCAAGCGTTATCCGGAATCAT1270.22423504069777705No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1170.20657873828062925No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1170.20657873828062925No Hit
GTGTCAGCAGCCGCGGTAATACGTGGGTCGCAAGCGTTATCCGGAATCAT1140.20128184755548495No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT1090.19245369634691104No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1080.19068806610519623No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1070.18892243586348145No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1060.1871568056217667No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT980.17303176368804846No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT920.16243798223775976No Hit
GTGTCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT820.14478167982061196No Hit
GTGTCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT810.1430160495788972No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC800.1412504193371824No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT760.1341878983703233No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC750.13242226812860852No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTGTTCGGATTCAT730.12889100764517894No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAT730.12889100764517894No Hit
GTGCCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT720.12712537740346416No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC690.12182848667831982No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT650.1147659657114607No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC620.10946907498631638No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC610.1077034447446016No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT610.1077034447446016No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC600.10593781450288679No Hit
GTGCCAGCCGCCGCGGTAACACGTAGGTGGCGAGCGTTATTCGGATTCAC600.10593781450288679No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC570.10064092377774247No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT570.10064092377774247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)