FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003939652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114598
Sequences flagged as poor quality0
Sequence length239
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC54384.7452835128012705No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC49664.333408960016754No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC49274.299376952477356No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC48834.26098186704829No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC48804.258364020314491No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC45253.94858549014817No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC45043.930260563011571No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC44513.8840119373811057No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC31042.7085987539049547No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC29482.5724707237473603No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC28232.463393776505698No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC27612.409291610673834No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT23472.048028761409449No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT21871.9084102689401212No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT21831.9049198066283881No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT21651.8892127262255884No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT15901.3874587689139426No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT15741.37349691966701No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT14841.2949615176530131No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT14291.2469676608666818No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT14101.230387964885949No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT14051.2260248869962826No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT13991.220789193528683No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT13851.2085725754376169No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC13841.2076999598596836No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC12701.1082217839752875No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC12041.0506291558316898No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC11831.0323042286950908No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT7440.6492259899823731No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT7020.6125761357091747No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6670.5820345904815093No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT6410.5593465854552435No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT6260.546257351786244No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6230.5436395050524443No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT6060.5288050402275781No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5630.49128257037644635No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT5360.46772194977224735No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT5060.4415434824342484No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTCAT5030.4389256357004485No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT4940.43107209549904885No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTCAT4590.40053055027138346No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTCAT4530.3952948568037837No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT4520.39442224122585035No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTCAT4430.38656870102445073No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT4260.3717342361995846No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC4080.3560271557967853No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3820.33333915077051957No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3760.3281034573029198No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC3680.3211225326794534No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT3390.2958166809193878No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3370.2940714497635212No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT3310.2888357562959214No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT3310.2888357562959214No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT3270.28534529398418823No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC3180.2774917537827885No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT3110.27138344473725545No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT3110.27138344473725545No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC3070.2678929824255223No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC3030.26440252011378906No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2980.26003944222412256No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC2920.25480374875652284No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2900.2530585176006562No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT2810.24520497739925654No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2730.23822405277579015No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2460.21466343217159115No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2420.21117296985985792No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2400.20942773870399134No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2380.20768250754812476No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2350.20506466081432487No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC2290.19982896734672506No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2250.1963385050349919No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2250.1963385050349919No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2220.19372065830119198No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT2120.18499450252185903No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC2060.17975880905425926No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC1990.17365050000872614No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC1970.17190526885285956No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1890.16492434422939317No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1760.1535803417162603No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1750.15270772613832703No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1740.15183511056039373No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATCAT1720.15008987940452712No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1700.14834464824866053No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1660.14485418593692734No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1620.14136372362519414No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1580.13787326131346095No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1570.13700064573552767No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1450.1265292588003281No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1450.1265292588003281No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1440.1256566432223948No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC1420.12391141206652821No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1410.12303879648859492No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1390.12129356533272832No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1350.11780310302099513No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1320.11518525628719525No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1300.11344002513132864No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1290.11256740955339535No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1280.11169479397546206No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC1270.11082217839752877No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1270.11082217839752877No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1220.10645910050786225No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC1200.10471386935199567No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC1200.10471386935199567No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATAAC1200.10471386935199567No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1190.10384125377406238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)