FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003939678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73913
Sequences flagged as poor quality0
Sequence length239
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC41645.633650372735513No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC38455.202061883565814No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC34634.68523805014003No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC32604.410590829759312No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC25863.4987079404164354No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC24663.3363549037381786No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC22132.9940605847415207No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC20172.7288839581670343No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC14441.9536482080283575No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC13151.7791186935992316No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC11781.593765643391555No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC10951.4814714596890939No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC9631.3028831193430113No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8801.1905889356405503No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8511.151353618443305No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC8431.1405300826647546No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC7871.0647653322149013No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC7461.0092947113498303No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC7421.003882943460555No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC7421.003882943460555No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC7310.989000581765048No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6940.9389417287892523No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC6820.9227064251214265No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC6770.9159417152598326No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC6710.9078240634259197No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC6470.8753534560902683No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC6360.8604710943947614No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC5970.807706357474328No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC5650.7644122143601261No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5340.722471013218243No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC5220.7062357095504174No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC5150.6967651157441858No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5130.6940592317995481No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTAC5090.6886474639102729No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC5050.6832356960209977No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC4930.667000392353172No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC4330.5858238740140435No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC4290.5804121061247682No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC4160.5628238604846239No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC4070.5506473827337546No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC4070.5506473827337546No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC3990.5398238469552041No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3760.5087061815918715No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3700.5005885297579586No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT3640.49247087792404587No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3630.491117935951727No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT3460.4681179224223073No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC3430.4640590965053508No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGATTTAC3380.4572943866437569No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC3370.45594144467143805No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT3290.4451179088928876No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC3080.4167061274741926No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT2930.3964119978894105No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2860.3869414040831789No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC2860.3869414040831789No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT2790.37747081027694723No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC2680.36258844858144035No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT2610.3531178547752087No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT2610.3531178547752087No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT2590.3504119708305711No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT2580.3490590288582523No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT2480.3355296091350642No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT2360.3192943054672385No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAC2260.3057648857440505No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT2250.30441194377173164No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT2160.29223546602086237No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAC2140.28952958207622476No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT2110.2854707561592683No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT1970.26652956854680504No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1940.2624707426298486No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1920.259764858685211No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1890.25570603276825454No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAC1870.25300014882361693No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1860.25164720685129816No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1750.23676484515579127No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1740.23541190318347247No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATGAC1740.23541190318347247No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATGAC1700.23000013529419724No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAC1690.22864719332187844No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1470.19888246993086467No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1390.1880589341523142No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATGAC1340.18129422429072017No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1310.17723539837376376No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATGAC1300.17588245640144495No Hit
GTGTCAGCCGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1280.17317657245680732No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1270.1718236304844885No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT1270.1718236304844885No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1250.1691177465398509No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1250.1691177465398509No Hit
GTGCCAGCAGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1250.1691177465398509No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAC1220.16505892062289448No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT1200.16235303667825687No Hit
GTGTCAGCAGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1190.16100009470593807No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1190.16100009470593807No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1150.15558832681666282No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC1110.1501765589273876No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT1090.14747067498275No Hit
GTGCCAGCCGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1060.14341184906579355No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT1060.14341184906579355No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1060.14341184906579355No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC1050.14205890709347474No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1050.14205890709347474No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1030.13935302314883716No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1020.13800008117651835No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1010.13664713920419955No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT990.1339412552595619No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC970.1312353713149243No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT970.1312353713149243No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC960.1298824293426055No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAC950.1285294873702867No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC900.12176477750869266No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC890.12041183553637386No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT870.11770595159173623No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC870.11770595159173623No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT850.11500006764709862No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC840.11364712567477982No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGATTTAC830.112294183702461No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT820.11094124173014219No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT790.10688241581318578No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT760.10282358989622935No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT750.10147064792391054No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC740.10011770595159174No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTTAT740.10011770595159174No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT740.10011770595159174No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT740.10011770595159174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)