FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003939808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003939808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68169
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC900113.203949009080374No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC58138.52733647259018No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC58098.52146870278279No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC49837.3097742375566614No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC39025.72400944710939No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT34765.099091962622306No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC33134.859980342971145No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC31334.595930701638575No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT22253.2639469553609413No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC21513.155393213924218No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT21373.134856019598351No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC20693.0351039328727136No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT14122.0713227420088307No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC13682.006777274127536No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC13371.9613020581202598No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTGGCAAGCGTTGTCCGGAATTAC12681.8600830289427743No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC9041.326115976470243No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTGGCAAGCGTTGTCCGGAATTAC8261.2116944652261292No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC8201.2028928105150434No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTGGCAAGCGTTGTCCGGAATTAC7661.1236779181152723No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC5580.8185538881309686No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTGGCAAGCGTTGTCCGGAATTAC5410.7936158664495593No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC5310.778946441931083No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC5100.7481406504422831No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC3600.5280992826651411No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC3540.5192976279540553No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC2810.4122108289691795No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC1980.2904546054658276No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC1550.22737608003638018No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC1150.1686983819624756No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1130.16576449705878038No Hit
GTGCCAGCCGCCGCGGTAATACGNAGGGTGCAAGCGTTAATCGGAATTAC1110.16283061215508515No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC1090.15989672725138993No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC830.12175622350335197No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT750.11002068388857106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)