FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003940104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003940104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88495
Sequences flagged as poor quality0
Sequence length239
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC50375.691846997005481No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC49435.585626306570993No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT41184.653370246906604No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT39884.506469292050398No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC38454.344878241708571No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC37064.187807220746935No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC36944.174247132606362No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC35774.042036273235776No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT32023.6182835188428726No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT29403.322221594440364No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC28743.2476411096672124No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC26633.009209559862139No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC18862.131193852760043No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC18732.116503757274422No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC14181.6023504152776993No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC13081.4780496073224476No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC12911.458839482456636No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC12621.4260692694502515No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC10291.1627775580541273No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC8881.0034465224023956No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC8050.9096559127634329No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC7970.900615854003051No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC6270.7085146053449347No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT6250.7062545906548393No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC6060.6847844510989322No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5930.6700943556133115No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC5920.6689643482682637No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC5680.641844171987118No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4950.5593536357986327No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC4940.558223628453585No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4900.553703599073394No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4890.5525735917283462No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4650.5254534154472004No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC4440.5017232612011978No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC4340.49042318775072036No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC4080.4610429967794791No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT3890.439572857223572No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3790.4282727837730945No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC3760.4248827617379513No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC3650.4124526809424261No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3550.4011526074919487No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3550.4011526074919487No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT3520.3977625854568055No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3320.3751624385558506No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC3300.3729024238657551No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC3060.3457822475846093No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT2970.3356121814791796No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC2900.3277021300638454No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2770.31301203457822474No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTAT2730.30849200519803377No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2600.2938019097124131No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC2320.2621617040510763No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT2210.24973162325555115No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2200.2486016159105034No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1960.2214814396293576No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1830.20679134414373695No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT1780.2011413074184982No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1770.2000113000734505No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC1660.1875812192779253No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1640.18532120458782983No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT1630.1841911972427821No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT1460.16498107237697046No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1410.15933103565173173No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1410.15933103565173173No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC1370.15481100627154076No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT1360.15368099892649303No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1340.15142098423639752No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1330.1502909768913498No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTCAT1310.1480309622012543No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1300.14690095485620655No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT1290.14577094751115882No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGATGCGAGCGTTATCCGGATTTAT1270.14351093282106334No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1250.14125091813096785No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1220.13786089609582464No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC1190.1344708740606814No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTCAT1180.13334086671563367No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT1180.13334086671563367No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC1170.1322108593705859No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1160.13108085202553815No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1150.12995084468049042No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1130.12769082999039494No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGTCTCAAGCGTTGTTCGGAATCAC1120.12656082264534718No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1080.12204079326515622No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT1050.118650771230013No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC1040.11752076388496525No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC1020.11526074919486977No Hit
GTGTCAGCAGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC1020.11526074919486977No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC1010.11413074184982203No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAT1010.11413074184982203No Hit
GTGTCAGCCGCCGCGGTAATACATAGGGAGCAAGCGTTATCCGGATTTAC990.11187072715972653No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC980.1107407198146788No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC970.10961071246963106No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTCAT950.10735069777953557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)