FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003940118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003940118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63246
Sequences flagged as poor quality0
Sequence length239
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC49297.793378237358885No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC43246.836796002909275No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC41936.6296682794168795No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC41496.560098662366No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC25714.065079214495778No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC23323.6871897036966765No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC23023.6397558738892575No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC21823.450020554659583No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC18592.939316320399709No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC17342.741675362868798No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC16982.684754767099896No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC16352.5851437245043165No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTGTTCGGAATTAC5120.8095373620466118No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTGTTCGGAATTAC4970.7858204471429023No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTGTTCGGAATTAC4670.7383866173354837No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTGTTCGGAATTAC4480.7083451917907851No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4320.6830471492268285No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4260.6735603832653448No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4130.6530057236821301No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC4120.6514245960218829No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2480.3921196597413275No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2400.3794706384593492No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2350.3715650001581128No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2180.3446858299339089No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT2090.33045568099168326No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2050.3241311703506941No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2040.32255004269044685No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2040.32255004269044685No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT2010.31780665970970495No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC1980.3130632767289631No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT1970.31148214906871585No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC1960.3099010214084685No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1950.3083198937482212No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1850.29250861714574833No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1840.29092748948550107No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT1820.2877652341650065No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC1780.28144072352401733No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1780.28144072352401733No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT1750.27669734054327544No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT1700.268791702242039No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1690.26721057458179176No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1670.2640483192612971No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAC1640.2593049362805553No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1600.25298042563956613No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1570.2482370426588243No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1560.246655914998577No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATTAC1540.2434936596780824No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1510.23875027669734056No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGAATTAT1340.21187110647313662No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1340.21187110647313662No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGAATTAT1340.21187110647313662No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1340.21187110647313662No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1320.20870885115264207No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1300.2055465958321475No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC1290.2039654681719002No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1270.2008032128514056No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC1260.19922208519115833No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC1240.19605982987066375No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1210.1913164468899219No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC1170.18499193624893273No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1160.18341080858868547No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTTGCAAGCGTTGTCCGGATTTAC1150.18182968092843815No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAC1120.1770862979476963No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT1120.1770862979476963No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGAATTAT1110.175505170287449No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGAATTAT1070.16918065964645984No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1040.16443727666571797No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1030.1628561490054707No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC990.15653163836448156No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC980.15495051070423424No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC970.15336938304398698No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC960.1517882553837397No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC930.14704487240299782No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC930.14704487240299782No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC900.14230148942225596No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC900.14230148942225596No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC880.13913923410176138No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGCTTTAC860.1359769787812668No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC840.13281472346077222No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC840.13281472346077222No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC840.13281472346077222No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT810.12807134048003038No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC790.12490908515953578No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC770.1217468298390412No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATTAC770.1217468298390412No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAC760.12016570217879391No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT760.12016570217879391No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC740.11700344685829933No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAC730.11542231919805204No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGCTTTAC720.11384119153780477No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAAC720.11384119153780477No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC700.11067893621731018No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATGAC700.11067893621731018No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC690.1090978085570629No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC690.1090978085570629No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAT690.1090978085570629No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC680.10751668089681561No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC680.10751668089681561No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC680.10751668089681561No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT670.10593555323656831No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC670.10593555323656831No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC670.10593555323656831No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATCAC660.10435442557632103No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATGCAAGCGTTATCCGGAATGAC660.10435442557632103No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAC650.10277329791607374No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTTAT640.10119217025582647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)