FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003940188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003940188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55887
Sequences flagged as poor quality0
Sequence length239
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT41897.495481954658508No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT40007.157299550879453No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC34596.189274786623007No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC33305.958451876107144No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT32365.7902553366614775No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT29475.273140444110437No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC25744.6057222609909285No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC24364.358795426485587No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC12162.1758190634673538No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC10901.950364127614651No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC10601.8966843809830551No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC10461.871633832554977No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC8671.5513446776531215No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC8251.4761930323688872No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC7921.4171453110741317No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTAC7891.4117773364109723No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5430.9716034140318858No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT5290.9465528656038077No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT5260.9411848909406482No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT4750.849929321666935No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT4550.8141428239125379No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3910.6996260310984665No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT3900.6978367062107467No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT3520.6298423604773918No Hit
GTGTCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3440.6155277613756329No Hit
GTGTCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC3240.5797412636212357No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT3120.5582693649685974No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT3110.5564800400808775No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2770.49564299389840216No Hit
GTGCCAGCCGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2770.49564299389840216No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT2710.48490704457208295No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT2610.4670137956948843No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT2600.46522447080716445No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2570.45985649614400487No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2540.45448852148084523No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC2460.44017392237908637No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT2380.42585932327732745No Hit
GTGCCAGCAGCCGCGGTAAAACGTAGGGTGCAAGCGTTGTCCGGAATTAC2370.4240699983896076No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC2060.36860092687029183No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT2040.3650222770948521No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGATTTAC1980.3542863277685329No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1860.3328144291158946No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT1850.3310251042281747No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1770.3167105051264158No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1740.3113425304632562No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAC1680.300606581136937No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1620.28987063181061784No Hit
GTGTCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1590.28450265714745826No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1490.26660940827025964No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1420.2540841340562206No Hit
GTGTCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1360.24334818472990144No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC1330.23798021006674183No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1300.23261223540358222No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC1280.22903358562814252No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1240.22187628607726306No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCAAGCGTTATCCGGATTTAC1200.2147189865263836No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1120.2004043874246247No Hit
GTGCCAGCAGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT1020.18251113854742604No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC950.16998586433338703No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC920.16461788967022742No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC920.16461788967022742No Hit
GTGCCAGCCGCCGCGGTAATACGGGGGATGCGAGCGTTATCCGGATTTAT920.16461788967022742No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC910.16282856478250757No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC860.15388194034390823No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC850.15209261545618838No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC820.14672464079302877No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC770.1377780163544295No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC770.1377780163544295No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT750.13419936657898976No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC750.13419936657898976No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC740.13241004169126988No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC740.13241004169126988No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCAAGCGTTATCCGGATTTAC730.13062071680355003No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC720.12883139191583015No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT720.12883139191583015No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC710.1270420670281103No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGATTTAC680.12167409236495072No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT670.11988476747723084No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATAAT650.11630611770179111No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT650.11630611770179111No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC610.10914881815091165No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCGAGCGTTGTCCGGAATTAC610.10914881815091165No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTAC600.1073594932631918No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTATCCGGAATGAT590.10557016837547194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAC590.10557016837547194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)