FastQCFastQC Report
Tue 26 Sep 2023
EGAF00003946526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003946526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35958749
Sequences flagged as poor quality0
Sequence length98
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC772680.21487955545950724No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT720600.20039629298560974No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG711050.19774047200585312No Hit
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC668830.18599924040739016No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA661670.18400806991366692No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT604360.16807036307075088No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG545660.15174610217947238No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA535490.1489178614083599Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA498830.13872284600334678No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG494230.1374436023900609No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT467630.13004623714801647No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA463840.12899225164924397No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT459720.1278464943260401No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC458800.12759064560338293No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT453960.12624465884505603No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG422930.11761532638412976No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC411230.11436159806338092No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC406470.1130378590200677No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA399330.11105225045509788No Hit
GCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTC389800.10840199140409473No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC387300.10770675030991761No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC382980.10650537369917958No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC375340.10438071691537434No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG362050.10068481525872884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)