FastQCFastQC Report
Sun 2 Apr 2023
EGAF00003946598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003946598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24212796
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC931070.38453634185824714No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT697770.28818233135900534No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT666330.27519746170578563No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT633080.26146505343703386No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC613540.25339494042736743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA607950.25108624381917727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC597510.2467744741251692No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT595370.24589064393884952No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG557310.2301716827746783No Hit
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA494820.20436301532462423No Hit
CTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAG479400.19799448192600308No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA468530.19350512018521115No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA457390.18890424715922935No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN452090.1867153219314283No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC437160.1805491608651888No Hit
CCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAG435950.18004942510563424No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA432610.1786699892073596No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA403410.1666102502164558No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT403250.1665441694548618No Hit
CCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGG398910.16475172879662472No Hit
GCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAA377100.1557440949818435No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG375880.15524022917468927No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC355180.14669103064346636No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG350390.14471273784324618No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG335990.13876546929978678No Hit
CCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTG335720.1386539580145969No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA333280.1376462264002885No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA331720.13700193897474708No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC319980.1321532630927878No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT319370.13190133018921069No Hit
ATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATT318480.1315337559528441No Hit
GAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAG315590.1303401721965526No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG315370.13024931114936086No Hit
CTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCG307000.12679246130847507No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA304530.1257723395513678No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT296350.12239396061487488No Hit
CCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTA294180.12149774028575634No Hit
GGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCT292170.1206676007182318No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC292050.12061804014703631No Hit
ACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTC286280.1182350026820529No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA283200.11696294802136854No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA283150.1169422977833704No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT282410.1166366742609982No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC273710.1130435328493248No Hit
GCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCT268080.11071831605073613No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT265400.10961146329403675No Hit
GCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAA264770.10935127029526041No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT264330.10916954820087692No Hit
CACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCA262820.1085459110133336No Hit
AATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATT260730.10768273106501207No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG257140.10620004397674683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG255480.10551445607520915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCC255140.10537403445682193No Hit
GGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAG252790.10440347327091014No Hit
GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCC247370.1021649874719136No Hit
CTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTT244300.10089706285882885No Hit
GTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGC243880.10072360085964463No Hit
CTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATC243060.1003849369564754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)