FastQCFastQC Report
Sun 2 Apr 2023
EGAF00003946600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003946600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24850071
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC943880.37982990068720524No Hit
GGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCT733300.29508970014612834No Hit
GTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGT687890.2768161105052778No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT639180.2572145568517692No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC625450.2516894217324369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA624770.2514157806631619Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTC619340.24923067624233347No Hit
GTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTT601830.24218441870850188No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG571960.23016433232725975No Hit
AGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAA519980.209246887061208No Hit
CTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAG492850.1983294132238093No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA479160.1928203746379638No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA471580.18977008154222175No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA447840.18021678891782644No Hit
CCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAG447210.1799632685154099No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC442170.17793510529607742No Hit
GGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACA412900.16615646691713679No Hit
GCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCT411680.16566552264579043No Hit
CCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGG405450.16315848755522672No Hit
GCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAA385050.1549492554769763No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG384470.1547158557414182No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC373130.15015248849792018No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN361160.14533560085200561No Hit
ATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGG354750.14275613136075144No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG349210.1405267614728344No Hit
CAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTA346710.1395207281299116No Hit
CCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTG345390.13898954252484833No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA342860.1379714367818104No Hit
CTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGC335040.13482456448514774No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT334660.13467164741702348No Hit
ATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATT327750.13189097125718474No Hit
GAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAG323980.13037387297605707No Hit
CAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTG321550.12939600856673608No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA320010.1287762920274956No Hit
CTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCG315550.12698152854372124No Hit
GGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTT307620.12379039077997No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC300410.12088899061898052No Hit
CCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTA299870.1206716874169092No Hit
GGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCT299460.12050669794866985No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA289550.1165187817773237No Hit
ACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTC289520.11650670937720861No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA287470.11568176203601188No Hit
CTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATT287400.11565359310241005No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC279930.11264756547375658No Hit
GCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCT278310.11199565586754259No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT276890.11142422892876241No Hit
GCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCT273050.10987896171403293No Hit
AATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATT270390.10880854223716302No Hit
CACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCA270010.10865562516903875No Hit
GCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAA266110.10708621315407912No Hit
CCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAG265560.1068648858186361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG265360.10678440315120227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCC261960.1054161978048272No Hit
GGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAG261140.10508621886834851No Hit
GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCC256700.10329950365131754No Hit
CTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTT255100.10265564231184691No Hit
GTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGC250940.1009816028292233No Hit
ATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTT249230.10029347602266409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)