FastQCFastQC Report
Mon 30 May 2022
EGAF00003946936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003946936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330637345
Sequences flagged as poor quality0
Sequence length98
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCT10544180.31890468997081983No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA10423890.3152665649429286No Hit
GCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAG8727650.26396443511243417No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT7437330.2249392003797998No Hit
ATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTG7408000.22405212575125172No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT5826510.17622056576821352No Hit
GAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTC5518710.16691127253033078No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA5450430.164846170053779No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG5290760.1600170119923991No Hit
GAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGG5015010.15167705874241155No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG4971790.15036988637807988No Hit
GGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAA4922540.14888033897078384No Hit
GACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGC4610530.13944371589361754No Hit
GCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCT4600460.13913915259632878No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA4489750.13579077100319686No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4459730.13488282758863793Clontech SMART CDS Primer II A (100% over 26bp)
GCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAA4241680.12828798876303582No Hit
GCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGC4240900.12826439796145833No Hit
GGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAA4220380.12764377841226615No Hit
AGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTT4186580.12662151034390867No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT3944130.11928870285357511No Hit
GACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATA3904750.11809766982008642No Hit
CTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGA3766190.11390697563216884No Hit
GGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGC3702390.11197736904160055No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA3636930.1099975563861366No Hit
GGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATC3636320.10997910716951831No Hit
CTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTAC3618290.10943379671766963No Hit
AAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCG3542050.10712794708655793No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC3423150.1035318620768625No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCG3368720.10188564755139805No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC3329980.10071397107304983No Hit
GTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATA3307110.10002227667295115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)