FastQCFastQC Report
Fri 23 Dec 2022
EGAF00003946942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003946942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321094411
Sequences flagged as poor quality0
Sequence length98
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22254880.693094592667949Clontech SMART CDS Primer II A (100% over 26bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG8873920.2763648228059628No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT7449170.23199313799329882No Hit
GCCCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTC7052920.21965253079412836No Hit
CCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGCCCCTCGG4455240.13875171436727374No Hit
GTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGT4398210.1369756012352392No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA4280180.1332997353230169No Hit
GTCGCGCCCGGCCGGCGCGCGGCCGGGCGCTTGGCGCCAGAAGCGAGAGC3803440.11845238875864458No Hit
CTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGA3314300.10321886294059476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)